FastQCFastQC Report
Wed 6 Sept 2023
SRR13794649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13794649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141141028
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA76417515.414266218891363Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC21687511.5365843870713483No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA18873331.337196580430178No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA12788300.9060653858919039No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC10849600.7687063183357287No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG10393820.7364137945771515No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATA7831350.5548599235085634No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA7246820.5134453179694851No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG6702000.47484421043043556No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT6154080.43602346441744777No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCG6055310.42902549923329164No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA5536690.39228069105462376No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG5476190.38799419825679604No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC5296970.37529626041833847No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG5095620.36103038728044407No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC4815320.34117081816918604No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4155620.2944303338927077No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA3902090.27646744928058764No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA3889930.27560589965378457No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA3768060.2669712735831852No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAG3675990.2604480109072183No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATC3451980.2445766513759557No Hit
TGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG3444680.24405943819539133No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA3424210.24260911575619246No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG3196230.2264564772760476No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3167570.2244258841589279No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG3064770.21714238895865204No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC3054580.21642041603948076No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG2978350.2110194351142178No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT2955310.20938702529501202No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA2932270.20775461547580623No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAG2926410.20733942790894228No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA2880990.20412137001014335No Hit
TTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA2874740.20367855050623548No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATAGATC2829810.20049520965654297No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2775540.1966501193402106No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA2770900.19632137014050938No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2671560.1892830198175969No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATTCTGTAGGCACCATCAAT2641270.18713693937385806No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG2569810.18207391829397757No Hit
ATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATCAATAGATCGGAAG2528810.17916902234834226No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2523600.1787998880098847No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG2518700.17845271751882097No Hit
TCAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATAGATC2504770.1774657614085112No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2487660.17625349873461316Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG2458020.1741534715192807No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT2386590.169092575973019No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT2310990.16373623125374998No Hit
ACCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2310360.16369159504775607No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAG2254310.1597203897367107No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT2247650.15924852127334654No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2228810.15791368616076681No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA2214780.15691964493839453No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2183750.15472113466539297No Hit
TAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGC2054650.145574254992673No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2025980.14354295336434703No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA2014540.14273241654439417No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2012690.14260134197123744No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1989340.14094696830463785No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGA1925060.13639265827084668No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1920170.13604619629098919No Hit
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1813790.12850905407887492Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAG1809380.12819660063691757No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT1766160.1251344152034942No Hit
ACGCAGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAATAGA1751480.1240943207527155No Hit
TGGGGAAGGTGGCTCGGGGGGGGCTGTAGGCACCATCAATAGATCGGAAG1750320.12401213345279022No Hit
AAAAAGCTCGTAGTTGGATCTTGGGCTGTAGGCACCATCAATAGATCGGA1717460.12168396562904445No Hit
TGGGGAAGGTGGCTCGGGGGGGGCCTGTAGGCACCATCAATAGATCGGAA1710030.12115754180279882No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA1680530.11906743374435391No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGAT1680150.11904051031851631No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCCTGTAGGCACCATCAATAGATCGGA1678820.11894627832808474No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1676070.11875143774636529No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG1657550.11743927499238563No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1657220.1174158941225793No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATAGA1626220.1152195093831965No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCA1608580.11396969561536707No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1584630.11227281127639228No Hit
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1577920.11179740025699685No Hit
ATCTCGCGCCGGGCCGTACCCATATCCGCAGCCTGTAGGCACCATCAATA1548600.10972004540026448No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1548180.10969028792960187No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGC1545660.10951174310562624No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCG1540590.10915252792405622No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCTGTAGGCACCATCAATAGATCGGAA1526230.10813510583187759No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG1513720.10724875831285571No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA1504640.10660543013757842No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1492610.10575309115645666No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTCTGTAGGCACCATCAATAGATCGG1457690.10327897002422287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)