FastQCFastQC Report
Wed 6 Sept 2023
SRR13794651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13794651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64288547
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1692048126.31958846417854Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA5045740.7848583045437316No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA4462890.6941967439394765No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA4186140.6511486408302244No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3904630.6073601259023633No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3282110.5105279483140286No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2749040.42760960206489035No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT2518440.39174007152471496No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA2496120.38826822450972487No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA2394120.3724022569681035No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT2154910.33519345210897367No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2099170.32652316749358173No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2094470.325792088597056Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA2008650.31244289904389966No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA1882080.29275509990916426No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATCG1878970.29227134344784617No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC1866690.29036120539479604No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1721550.2677848668752772No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT1652580.25705667294051615No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1609500.25035563488470197No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1580420.245832278648326No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1554530.24180512276937913Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT1548940.24093560552861773No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1376370.21409256613001382Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1364120.21218709453800533No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1348630.20977764515349834No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1318340.20506607498844234No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGATC1211670.18847369501133693No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC1205430.18750307111467304No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC1196410.18610002182814928No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1154470.1795763092919179No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA1121990.1745240874708212No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1115370.1734943550676297No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1093850.17014694701375038No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1087130.16910165974042002No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1023640.15922587268926766No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA994860.1547491810633082No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT981220.1526274967763698No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG961980.14963473976165614No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT955260.14858945248832578No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAGA955030.14855367628700647No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATAG943640.14678197657819206No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCAGTCAC943110.1466995357664562Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA884920.1376481568326626No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG873600.13588734553294537No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG871150.1355062512145437No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATAGA867470.13493383199343423No Hit
CTGGATTGAGGCTCCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC844470.13135621186149998No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA835270.12992516380872632No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC829070.12896076186011796Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGATCGGA827710.12874921562622965No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG819720.127506381502136No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT816090.12694173971609593No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC807340.12558068857894703No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGACTCCAGTCAC805180.125244703383948Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGAT803510.1249849370526293No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT767810.11943184841306181No Hit
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC764910.11898075717903533Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAAGCTCGTAGTTGGATCTTGGCTGTAGGCACCATCAATAGATCGGAA764570.11892787062056326No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT762420.1185934409125781No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATAGATCG747640.11629443110605688No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCGTCAC742410.1154809113977953Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG736660.11458650636481177No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAATA724160.1126421475974562No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA694160.10797568655580285No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA681340.10598155220400299No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG667890.1038894221703284No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA662880.1031101231763723No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA657590.10228727054602743No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT655820.10201194934456989No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT652950.10156552457158505No Hit
TTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATAGATCGG650280.10115020953887789No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG649490.10102732606478103No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA648270.10083755664908713No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)