FastQCFastQC Report
Wed 6 Sept 2023
SRR13794652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13794652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77793939
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2391235530.738069452942856Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA11162231.434845714651369No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA9857131.2670820023652485No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA9653121.2408575943172129No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG7919581.0180201827805635No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA7421650.9540139110323235No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC7327270.9418818604878717No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG5902760.7587686233499502No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG5567490.7156714355343287No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG4975710.6396012419425118No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA4929970.6337216065123017No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4881960.6275501745707979No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA4498110.5782082843240525No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA4242390.545336828875576No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA4166430.5355725720483185No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA4100990.5271606056610657No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT3046210.3915742073428112No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2973150.3821827301995854Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT2924820.3759701639481194No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC2581090.3317854878128745No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2523470.32437874112532084Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2452990.31531890935616463No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2180490.2802904735290496No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG2069280.26599501537002773No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1850750.23790413800746096Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1827240.2348820516724317No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1803000.23176612769280133No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA1607570.20664463333062488No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT1605970.2064389617808143No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1595470.20508924223518235No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGAT1584190.20363925780901773No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1556540.2000849963388536No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT1415200.18191648580746117No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1371090.17624637826862063No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCAGTCAC1346120.1730366166443892Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1324130.17020991828168003No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC1262380.16227228190617782No Hit
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1198530.1540647016215492Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATA1145000.14718370283319887No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGACTCCAGTCAC1137960.1462787480140323Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG1136360.1460730764642217No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1123060.14436343170642124No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1116700.14354588729592416Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAATA1062470.1365749072045317No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT1057540.13594118174167785No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT1030750.13249746872953688No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG1029070.13228151360223575No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1007860.12955508012005923No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1003030.12893420912906853No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA983370.126407019960771No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG961250.12356361078463965No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG954890.12274606637414258No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT952270.12240927921132777No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATAGATC945730.121568596751477No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA945640.12155702772680016No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAATAG944290.12138349235664748No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC897320.11534574692252052No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT888810.11425183136696548No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG866140.11133772259558677No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT862460.11086467803102244No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA854830.10988388182786321No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA852010.10952138572132206No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT848060.10901363408272718No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAGAT840360.10802383974926376No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG824140.10593884441305895No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA814180.10465853901548809Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCCCCATCAATAGATCGGAAGAGCCCACGTCTGAACTCCAGTCA791340.101722577641942Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACCCAGTCAC789370.10146934454623771Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph