FastQCFastQC Report
Wed 6 Sept 2023
SRR13800639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13800639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116995433
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2853714224.391671767221887Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA55753684.765457810648044No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA49080394.195068879312579No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG48859704.176205749843244No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA35401563.0258924722300913No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA25890572.212955611694689No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG24824792.1218597481493147No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC20134901.7209988017224571No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT16707121.4280147157539047No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG15948861.363203638897597No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT14178241.2118626886914465No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA8065970.6894260564854698No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7139940.6102751036444304Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG6666750.5698299351565287No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG6062350.5181697989869399No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT5699150.48712585216894744No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA5441450.4650993513567321No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA5436690.4646924978686988No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC5257270.44935685651934804No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT4790500.40946042739976013No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAG4642610.3968197630415197No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT4433230.378923337973372No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4064760.34742894622219994No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAGA3969690.339302988006378No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACCCCAGTCA3880110.33164627887654385Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT3761660.3215219520577354Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT3615850.3090590724169549No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA3563450.30458026511171593No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA3474270.296957745350624No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA3452030.2950568164485532No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT3368300.2879001268365749No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3354930.2867573471863641No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA3184710.2722080613180858No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA3001490.25654762096568334No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT2960590.253051758011785No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT2947860.25196368135156183No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG2765230.23635367031805424No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCAA2563680.21912650214303667No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2472700.2113501302226045No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2442140.20873806245069412Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCAA2403180.2054080179351958No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA2311620.19758207143008738No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG2298370.19644954859049926No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA2224870.19016725208410484No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2166600.18518671579257287Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAG2044600.17475895832617672No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2013010.17205885292975498No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2006380.17149216414285162No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT2006150.17147250525582483No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGAT2000230.17096650259843904No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGATC1970520.168427087235106No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1965900.16803220002613264No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAATA1930450.16500216722134786No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA1921230.16421410227183825No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGG1887470.1613285195499896No Hit
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1828280.15626934771035036Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1794910.15341709962302547Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA1784630.1525384328463488No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCAAT1784430.15252133816197766No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1495720.12784430653801673Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG1391130.11890464134612844No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGA1385670.11843795646279628No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1359240.11617889392314998Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATC1305090.1115505081296635No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT1298650.11100005929291273No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG1262040.10787087731877534No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG1255320.107296495923905No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1234860.10554770971273725No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG1209210.10335531644213838No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1198530.10244246029671945No Hit
CTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1177770.10066803205899498Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)