FastQCFastQC Report
Wed 6 Sept 2023
SRR13800640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13800640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114665938
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1369707611.945200326185795Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA69814916.088548283623687No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA68622135.984526110971158No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA55811864.867344302368154No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG42799813.7325652889177956No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT25897822.2585451662201548No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG22621241.9727950945641763No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC22565401.9679252961764462No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG20260181.76688739074371No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT18073321.5761716439279465No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT17479011.5243419541032315No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT8740370.7622464135775002No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT7932830.6918209660483482No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC7294490.6361514262413307No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7088100.618152183955448No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAGA6866360.5988142703720786No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA6792540.5923764387642301No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT6439380.5615774058378173No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA6393540.5575797060152248No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6213080.541841815308745No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA6101580.5321179163074565No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT5771020.5032898261382557No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG5497630.47944752346594854No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA5151230.44923802916956906No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG5126950.4471205738534141No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG4923360.42936551916577004No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA4240940.36985176888362437No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4165190.3632456222527042No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT3987390.347739709764551No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA3872290.3377018552798129No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA3820130.3331529891640532No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3305510.28827305280492277Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA3129400.27291452497427793No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAG2970460.25905339037997493No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGG2555400.22285606733535812No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT2480940.21636242141934076No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA2428170.21176035729110768No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA2426550.21161907732355534No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGAT2349030.20485856924660575No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA2252230.19641665513607012No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCAA2248070.1960538621329727No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCAA2223700.1939285579297315No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2077900.18121336085001982No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC1965990.17145370580756075No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1943610.16950194921878195Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1896260.16537256251285365No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG1882460.16416906649296323No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCTGTAGGCACCATCAATAG1873390.1633780730943831No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAG1861200.1623149849434799No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1766000.15401260660336638No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT1758940.15339690501637895No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATC1694620.14778756704541152No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG1683600.14682651442662947No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA1674720.14605209090078694No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGA1665010.14520528319403797No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1535200.13388457171998192No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAATA1517820.13236886441377213No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC1453090.12672377040163402No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1435990.12523248185524807No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG1423370.12413189346604395No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG1389480.12117635142879135No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1371080.11957169006893748No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAG1362610.1188330225842656No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAG1343090.1171306861851163No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAG1340980.11694667338787218No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1279220.11156059264958004Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG1243550.10844981706773288TruSeq Adapter, Index 11 (100% over 35bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG1218010.10622247733237049No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT1214690.10593294060874468No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC1203520.10495880651148558No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGCACCATCAATA1191940.1039489163730558No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA1179920.10290065389775993No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1161130.10126198069386569No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)