FastQCFastQC Report
Wed 6 Sept 2023
SRR13800641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13800641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108569546
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4027142537.09274514236248Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA32972403.036984238655654No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA24042052.214437739290169No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG21336041.9651956544057023No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA21229561.9553881159270943No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG13308541.2258078338100447No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10899271.0038975386339002Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC10881771.0022856685796586No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG9574190.8818485802639352No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT8545530.7871019374070146No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7911420.7286960562587229No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC7728900.7118847121272848Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5220990.48088899625683246Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4593190.42306430939667006Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA4302280.396269502683561No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3556090.3275402846393039Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC3550670.32704106545679024No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG3471870.31978304486969117No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC3322590.30603333277270955No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3273610.3015219387580381No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG3086800.28431545619616017No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3030620.2791408927877436No Hit
CACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCA2999060.2762340002784943No Hit
CTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2957620.2724170919900503Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2892740.2664411988975251Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2703620.24902194948848733No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA2607180.24013916388671278No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAGA2562360.23601093441064955No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2476660.2281173764878781Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA2393530.22046053319592956No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA2280770.21007456363499946No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2265260.20864598623264022No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2137040.19683604461236304No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2043760.1882443166889544No Hit
CTGTAGACACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1973070.18173328273842096Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG1912960.17619673936925187No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1832640.16879871635458438Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA1746070.16082502546340205No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1689740.15563664602594912No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1678600.15461057560284908No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT1656240.1525510662078296No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT1574370.14501027755978643No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1565340.1441785526117978No Hit
CTGTAGGCACCATCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1555220.14324643118614497Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1490000.13723922176113731No Hit
TACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAG1334490.12291568392484573Illumina Single End PCR Primer 1 (100% over 21bp)
CTGTGGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1333000.12277844470308459Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA1304870.12018747872446663No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1290320.11884732390794008No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGG1267090.11670768154450972No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1202970.11080178966576872No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGAT1178240.1085239870119748No Hit
CCACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGC1128120.10390759117662701No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)