FastQCFastQC Report
Wed 6 Sept 2023
SRR13800643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13800643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118095736
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA64853285.4915852338648365No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA61948825.2456440933650645No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA54011914.573569870465095Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA49905424.22584436071426No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG46318273.9220950365218945No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG27166752.3004005834723786No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC20485881.7346841379607472No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT17453471.477908567333879No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG16128561.3657190806618116No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG15340801.2990138780285851No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT13140791.1127234940980426No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG10987720.9304078514739941No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT8003640.6777247232702797No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT7768930.6578501699671866No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA7202270.6098670658185322No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA7085390.5999700107715997No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6982100.5912237170019415No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA6654440.563478430753842No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT6137320.5196902282737794No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC5995470.507678786980082No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA5849990.4953599679500706No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAGA5712160.4836889284470017No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT5532780.46849955700348067No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA4184490.3543303206137773No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4095110.34676188478134384No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC4037490.3418827924490009No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA3772510.31944506446871207No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT3737730.3164999962403384No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3707040.31390125719695755No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG3134520.265421945462959No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2858050.2420112780363213No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2779510.2353607415597122No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGAT2682420.22713944557659557No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAATA2602720.2203906837076658No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2291700.19405442377699395No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA2192130.18562312867926067No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATA2122680.17974230669937144No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT2105690.17830364341012278No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGA2082250.1763188130687462No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1918170.16242500067911006No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1885470.15965606074041488No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1820710.15417237418292562No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG1775330.15032972909369058No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1771500.15000541594490763No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAA1763290.14931021726305174No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG1722480.14585454634873524No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA1710400.1448316474356026No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1697260.1437189908363838No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGG1679870.14224645672219696No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCAT1667590.14120662239659526No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAG1655680.1401981185840613No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA1643890.13919977601900885No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGAT1621490.13730300982247148No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCA1617330.13695075324311454No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA1588730.1345289892600356No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1582500.13400145116162365No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCA1531760.12970493701821717No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1528790.12945344614305127No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGAT1426750.12081299870132484No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCTGTAGGCACCATCAATAG1422930.12048953232316534No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA1411970.11956147171985956No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG1384380.11722523156974948No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1377980.11668329837073882Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1371230.11611172819990724No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG1369440.11596015625830894No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT1284830.10879563001326313No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT1284330.10875329148209044No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAATA1245950.10550338582927331No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCAT1215950.10296307395891076No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA1194810.10117300086092862No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)