FastQCFastQC Report
Fri 1 Sept 2023
SRR13808052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12764689
Total Bases351.4 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCTGTAGTCCAACGGTTAGGATAATTGC662640.5191195805867264No Hit
GAGAATTCCACGTTGTGGCCGTGGAGACCTGGGCT359340.2815109713993032No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT338750.2653805353189569No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT326710.2559482647795023No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA319780.2505192253410953Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGTCTGTAGTCCAACGGTTAGGATAATT291020.227988319966119No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT272360.21336986745231318No Hit
GGGGCTATAGCTCAGCTGGTAGAGCGCTTGAAT269720.21130166195196765No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCT267430.20950765036265281No Hit
GGGTGTTTGGTCTAGTGGTATGATTCTCGCT264100.20689889115198967No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCC252850.19808551544029No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC246740.1932988731648691No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGC203450.15938500342624878No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT198890.1558126484711065No Hit
GGGAGACCATCGACTACGCCTTTCGGCCTGATC158810.12441352860222447No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTTGGCT158270.12399048656806287No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT148890.1166420897524413No Hit
GAGAATTCCACGTTGTGGCCGTGGAGACCTGGGC138500.10850244765070265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)