FastQCFastQC Report
Fri 1 Sept 2023
SRR13808055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4897552
Total Bases138.5 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1910763.901459341319909No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC823421.6812889378203644No Hit
TCAGTGCACTACAGAACTTTGT684971.3985966866712185No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA452670.924278088318409Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT316930.6471192138439776No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC315730.6446690101503773No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTG280320.5723675828250522No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC238480.4869371473748518No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGA161050.3288377540452863Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGAGGTAGTAGTTTGTGCTGTT155320.3171380314083444No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT151700.30974658359931656No Hit
TTCAAGTAATCCAGGATAGGCT133800.27319771183644403No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA133770.273136456744104No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC129060.26351940724672246No Hit
CAGAGTTCTACAGTCCGACGATCCAG125080.2553928983296145Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGC121360.24779726687945325No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT119360.24371359405678591No Hit
GGGTGTTTGGTCTAGTGGTATGATTCTCGCT106240.2169247003400883No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA105600.21561792503683475No Hit
TCAGTGCACTACAGAACTTTGC100960.20614380408824654No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA96440.19691470350901838No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATT92150.188155225304397No Hit
TCCGTCCAAGGCTAAATACATACCCGCTG85640.17486287026661482No Hit
TGAGGTAGTAGGTTGTATGGTT84610.17275977876294116No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT83220.16992162615118736No Hit
GAGAATTCCACGTTGTGGCCGTGGAGACCTGGGCT81760.1669405449906402No Hit
CTCCGTCCAAGGCTAAATACATACCCGCTG80000.16334691290669298No Hit
AGAGTTCTACAGTCCGACGATCAG75740.15464868979441157Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACCCGTAGATCCGATCTTGT70950.14486829338412333No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT67260.13733391702630213No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC65800.13435283586575497No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCT60160.12283687850583311No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG59840.12218349085420634No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT57740.11789563439040567No Hit
ACCTCCTGGGAAGTCCTCGTGTTGCATTCC56040.11442451249113843No Hit
TCGTACCGTGAGTAATAATGC55780.11389363502419167No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTA55270.11285229845441151No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATT54690.11166803333583798No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT52930.10807440125189073No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT50770.10366403460341003No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTAC50710.10354152441873002Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCATAGTCAAAATCTGAGTTTGATC50080.10225516747958979No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC49050.10015207597591613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)