FastQCFastQC Report
Fri 1 Sept 2023
SRR13808056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5287284
Total Bases145.6 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT924921.7493291451716988No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC255030.4823459454797586No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA251130.4749697576298152No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT243080.4597445493754449No Hit
GAGAATTCCACGTTGTGGCCGTGGAGACCTGGGCT175300.331550187203865No Hit
GGGTGTTTGGTCTAGTGGTATGATTCTCGCT147740.2794251263975984No Hit
TCCGTCCAAGGCTAAATACATACCCGCTG140400.26554276259796145No Hit
TCTCGGTAGCTAGTCGTCGTGGTTGGGACTG132780.2511308263373029No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC116110.21960235160433977No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCT115820.21905386584113884No Hit
CTCCGTCCAAGGCTAAATACATACCCGCTG107210.20276951266472543No Hit
GCGTCTGTAGTCCAACGGTTAGGATA92340.17464543232404386No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA86350.16331636431861804Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGAATTCCACGTTGTGGCCGTGGAGACCTGGGC83450.15783150668660884No Hit
TCCGTCCAAGGCTAAATACATACCCGCTGAG83420.15777476678007082No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT83050.15707497459943517No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCC82980.15694258148417978No Hit
TCTCGGTAGCTAGTCGTCGTGGTTGGGACT81190.15355710039407755No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC79780.15089032478679035No Hit
TCCGTCCAAGGCTAAATACATACCCGCTGAGT69650.13173114967911692No Hit
TGCAAATCTAACGAAGTGCTCCTGGAC67300.12728652366697155No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT65030.12299320407226091No Hit
TGAGAATTCCACGTTGTGGCCGTGGAGACCTGGGC65000.1229364641657229No Hit
CCGTCCAAGGCTAAATACATACCCGCTGAG64220.12146122659573422No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT63120.1193807633560066No Hit
AACTCCGTCCAAGGCTAAATACATACCCGCTG62390.11800009229691463No Hit
ACCTCCTGGGAAGTCCTCGTGTTGCATTCC55000.10402316198638091No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATT54530.10313423678395184No Hit
TCATAGTCAAAATCTGAGTTTGATCCTGGCTC54430.10294510376215843No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGC53370.10094029373114817No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA53010.10025941485269185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)