FastQCFastQC Report
Fri 1 Sept 2023
SRR13808057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8615229
Total Bases242.4 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1901652.2073121910050215No Hit
GTCAGGATAGCTCAGTTGGTAGAGC1835502.1305295541186426No Hit
GGGGATGTAGCTCAGATGGTAGAGT1553261.8029236367367598No Hit
GTGAAACCGTGCTGAGCTCCCAAGC1081131.2549057024485362No Hit
GCCCGTCTAGCTCAGTTGGTAGAGC804620.9339507980577184No Hit
GGGGATGTAGTTCAGATGGT633470.7352909597643893No Hit
CGTGAAACCGTGCTGAGCTCCCAAGC577230.6700112092203236No Hit
TGAAACCGTGCTGAGCTCCCAAGC536700.6229666094772408No Hit
GCGCTCTTAGTTCAGTTTGGTAGAACGCGGG487480.5658352203986684No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA473110.5491554548346887Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCCGTCTAGCTCAGTCGGTAGAGC466340.5412972771820691No Hit
GGGGATGTAGCTCAGATGGTAGAGA325020.3772621714408288No Hit
GGGGGTGTAGCTCATATGGTAGAGC303440.35221350471357177No Hit
CCCGTCTAGCTCAGTTGGTAGAGC299490.3476286004701674No Hit
AGCGGAGTAGAGCAGTTTGGTAGCTCACGAGG296680.3443669344134671No Hit
TCCGTTGTAGTCTAGTTGGTC290980.33775074347994694No Hit
GGGGGTGTAGCTCAAATGGTAGAGC284870.33065865109331394No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC278790.32360138076422573No Hit
AGGGCTATAGCTCAGTTCGGTAGAGC278460.3232183381312325No Hit
GGGGATGTAGTTCAGATGGTAGAAC248230.2881293114785457No Hit
CGTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGA240980.27971398090520866No Hit
GAAACCGTGCTGAGCTCCCAAGC240090.2786809265313783No Hit
GCGCTCTTAGTTCAGTTTGGTAGAAC238990.2774041177547341No Hit
GTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGA232330.2696736209797789No Hit
CCGACCTTAGCTCAGTTGGTAGAGC227950.2645896005782319No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC227470.2640324476575144No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGA221730.25736982731393443Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTGGGTAGTGAAATAGAACGTGAAACCGTGC207230.24053916616726032No Hit
GGGGACGTAGCTCATATGGTAGAGC202430.23496763696008544No Hit
GCCCGTCTAGCTCAGTTGGTAGAG199650.23174079296093003No Hit
AAACCGTGCTGAGCTCCCAAGC197330.22904788717746216No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGG196330.2278871519259674No Hit
AACCGTGCTGAGCTCCCAAGC195070.22642462550908396No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT191140.22186293597070955No Hit
GTCAGGATAGCTCAGTTGGTAGAGT183940.21350564215994722No Hit
AGTTCAGTTTGGTAGAACGCGGG181980.2112306010670175No Hit
GTCAGGATAGCTCAGTTGGTAGAG174830.2029313440188299No Hit
TGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA173250.20109738232146818No Hit
GTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA164430.19085969740328435No Hit
GAGTTCTACAGTCCGAGATCGAGTTCTACAGTCCG159940.18564799612407284No Hit
TGTAGTCTAGCTGGTCAGGATATTCGG159070.1846381564552724No Hit
CAGAGTTCTACAGTCCGACGATCCAG153210.17783624788151306Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCTCGTGTCACCAGTTCAAATCTGG153090.1776969596513337No Hit
ACCGTGCTGAGCTCCCAAGC151090.17537548914834417No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTG146990.17061647461721563No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT144470.1676914217834488No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC143810.16692533651746227No Hit
GGGGACGTAGCTCATATGGT142610.16553245421566856No Hit
GCTCTTAGTTCAGTTTGGTAGAACGCGGG137920.16008860588615811No Hit
TGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGA136010.1578716015558031No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC135360.15711712364233152No Hit
AGAGTTCTACAGTCCGACGATC132380.15365813259287708Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCCGTCTAGCTCAGTCGGTAGAGC129970.15086076063677473No Hit
ACCTCCTGGGAAGTCCTCGTGTTGCATTCC128840.1495491298025856No Hit
AGCGAGTACCCGCCCGGCCTGGAAGACA128600.14927055334222689No Hit
CGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA127500.14799374456558265No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCC127490.14798213721306772No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAGC118880.13798820669769776No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA118200.13719890672668134No Hit
GTGGGTAGTGAAATAGAACGTGAAACCGTGC117610.13651407292829942No Hit
GGGCCTGTAGCTCAGAGGTTAGAGCACGTG107420.12468618071556775No Hit
AGAGTTCTACAGTCCGACGATCAG102500.11897536327821351Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTG101230.11750122950881514No Hit
CCGACCTTAGCTCAGTTGGTAGAG100990.1172226530484564No Hit
TTGTAGTCTAGCTGGTCAGGATATTCGG100270.11638692366738018No Hit
GTGAAACCGTGCTGAGCTCCCAAGT99800.11584137809917763No Hit
GCGCCCGTGGCTCAACGGATAGAGCATCTGACT96670.11220827676199902No Hit
ACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA94880.11013056066182339No Hit
AGAACGTGAAACCGTGCTGAGCTCCC93880.10896982541032862No Hit
TGGTCGTGGGTTCGAGCCCCACGGTGGGC93790.10886535923769408No Hit
GCCTGTCTAGCTCAGTTGGTAGAGC91620.10634656374195045No Hit
CGTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA87270.10129736539794823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)