FastQCFastQC Report
Fri 1 Sept 2023
SRR13808058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8886163
Total Bases243.4 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGATAGCTCAGTTGGTAGAGC2068942.3282714935568927No Hit
GGGGATGTAGTTCAGATGGTAGAAC1210471.3621964845794523No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGG936711.0541220096907968No Hit
GCCCGTCTAGCTCAGTTGGTAGAGC922141.0377257315671569No Hit
AGGGCTATAGCTCAGTTCGGTAGAGC876740.9866350639753062No Hit
AGCGGAGTAGAGCAGTTTGGTAGCTCACGAGG823760.927014280516799No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC783460.8816628729407732No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT675940.7606657676659768No Hit
TGTAGTCTAGCTGGTCAGGATATTCGG637040.71688984323155No Hit
GCCCGTCTAGCTCAGTCGGTAGAGC614580.6916145922598989No Hit
GGGGACGTAGCTCATATGGTAGAGC518860.5838965591785791No Hit
GCGCTCTTAGTTCAGTTTGGTAGAAC462510.5204833627292229No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCC456930.5142039370648501No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA428570.48228914999646083No Hit
TTGTAGTCTAGCTGGTCAGGATATTCGG423730.4768424797069331No Hit
GGGGGTGTAGCTCATATGGTAGAGC418610.4710807127890857No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC415210.46725453944520257No Hit
GCGCTCTTAGTTCAGTTTGGTAGAACGCGGG392240.44140536247196904No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC352890.39712303274202826No Hit
GTGAAACCGTGCTGAGCTCCCAAGC330930.3724104543209482No Hit
GTCAGGATAGCTCAGTTGGTAGAG328270.36941703635191026No Hit
GGGGGTGTAGCTCAAATGGTAGAGC301020.3387513823457886No Hit
GCCCGTCTAGCTCAGTTGGTAGAG285470.3212522660230293No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCG272870.3070729177486391No Hit
GGGGATGTAGTTCAGATGGT271090.3050698034686062No Hit
CCTCGTGTCACCAGTTCAAATCTGG264110.2972148946626345No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT230120.25896441467481524No Hit
TCCGTTGTAGTCTAGTTGGTC228100.2566912175705082No Hit
CGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA224320.2524374130881911No Hit
CCGACCTTAGCTCAGTTGGTAGAG222610.25051307296523817No Hit
GCGCTCTTAGTTCAGTTTGGTAGAACG215530.24254562964915227No Hit
CCGACCTTAGCTCAGTTGGTAGAGC195630.2201512621364249No Hit
AGTGGGTAGTGAAATAGAACGTGAAACCGTGC195590.2201062483323792No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT189990.21380431576598358No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGC179300.20177437663477477No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCTGG166990.18792137843971576No Hit
CCCGTCTAGCTCAGTTGGTAGAGC166190.1870211023588021No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCC162870.18328495662301042No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT152190.171266270942813No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTG150000.16880176517131185No Hit
GGGGATGTAGCTCAGATGGTAGAGCGCTCGC146370.16471676245416608No Hit
TGGGGCGTGGCCAAGTGGTAAGGCAGCGGG141190.15888747483025012No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT134800.15169651963395225No Hit
GTCAGGATAGCTCAGTTGGTAGAGT132660.1492882811175082No Hit
GGGCCTGTAGCTCAGAGGTTAGAGCACGTG132480.14908571899930262No Hit
GGGGATGTAGCTCAGATGGTAGAGCCTCGC126600.14246868980458718No Hit
TCCATTGTCGTCCAGTCCGGTTAGGATACCTGG123050.13847371469553282No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTC122470.13782101453687043No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCG121960.13724708853528794No Hit
GTCAGGATAGCTCAGTTGGTAG121490.13671817633775116No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCC119770.1347825827637868No Hit
GGGGATGTAGCTCAGATGGTAGAGT118900.1338035325257932No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTC108410.12199866241481279No Hit
GCCTGTCTAGCTCAGTTGGTAGAGC106650.12001805503680273No Hit
AGCAAGGTCGGCTTCGTGTACCGCGAGA106560.11991677397769993No Hit
TCCATTGTCGTCCAGTCCGGTTAGGATACCT105530.11875766852352361No Hit
GCGTCTGTAGTCCAACGGTTAGGATA99850.11236570834903659No Hit
GTGGGTAGTGAAATAGAACGTGAAACCGTGC98410.110745211403392No Hit
CCGACCTTAGCTCAGTTGGTAG98200.11050888893215216No Hit
TGTCGTCTAGTCCGGTTAGGATATCTGG97690.10993496293056969No Hit
TTGTCGTCTAGTCCGGTTAGGATATCTGG97200.10938354383101007No Hit
GGGTGTTTGGTCTAGTGGTATGATTCTCGCT95760.10776304688536549No Hit
AGGTCTGTAGTTCGATCCTGCATGGGG94700.10657018107815487No Hit
CCTCGTGTCACCAGTTCAAATCTG93780.10553486358510418No Hit
GGGGATGTAGCTCAGATGGTAGAGCTCGC93470.10518600660375012No Hit
GCTCTTAGTTCAGTTTGGTAGAACGCGGG91540.10301409055854592No Hit
GGGCATTTGGTCTAGTGGTATGATTCTCGCTT90560.10191125235942666No Hit

[OK]Adapter Content

Adapter graph