FastQCFastQC Report
Fri 1 Sept 2023
SRR13808059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14735496
Total Bases420.6 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT9339276.33794071132726No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC1375630.933548487271823No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA790340.5363511347022184Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT753790.5115470833150102No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC598870.4064131943709259No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA591210.4012148623975738No Hit
GCCCGTCTAGCTCAGTCGGTAGAGC578280.39244013231722913No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG561190.38084228722263574No Hit
GTCAGGATAGCTCAGTTGGTAGAGC558240.37884031864282003No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATT429160.29124231719108745No Hit
GCCCGTCTAGCTCAGTTGGTAGAGC409280.27775108486338024No Hit
TTCAAGTAATCCAGGATAGGCT399620.27119548605625493No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTG371770.2522955453959609No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC348750.23667340413922952No Hit
GCGCTCTTAGTTCAGTTTGGTAGAACGCGGG317660.21557469120822267No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGA310720.2108649752950291Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATT304200.20644028541692794No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT297910.20217168122471074No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC285310.19362090017193856No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTA262770.1783245029553128No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC256860.17431377946151252No Hit
TGAGGTAGTAGTTTGTGCTGTT249380.16923760150320016No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCC247640.16805677935781735No Hit
CAGAGTTCTACAGTCCGACGATCCAG241130.16363887581388506Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATA240810.16342171312048132No Hit
GCGCTCTTAGTTCAGTTTGGTAGAAC240050.16290595172364744No Hit
TCGTACCGTGAGTAATAATGC238470.16183371092496648No Hit
GGGTGTTTGGTCTAGTGGTATGATTCTCGCT233710.1586034158605859No Hit
GAGTTCTACAGTCCGAGATCGAGTTCTACAGTCCG203990.13843443071071376No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCT198000.13436941654356255No Hit
CTAGACTGAAGCTCCTTGAGG196740.13351433843828533No Hit
TCAGTGCACTACAGAACTTTGT195210.13247602931044872No Hit
AGAGTTCTACAGTCCGACGATCAG182250.12368094022759736Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGGGGTGTAGCTCATATGGTAGAGC175970.11941912236954902No Hit
TAGCTTATCAGACTGATGTTGAC163280.11080726430925704No Hit
TGAGGTAGTAGATTGTATAGTT148040.10046489103590404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)