FastQCFastQC Report
Fri 1 Sept 2023
SRR13808061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22542044
Total Bases614.7 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT5616212.491437777337317No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC891210.3953545650075033No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC541600.24026215191488404No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA478040.21206595107346962Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGGTGTTTGGTCTAGTGGTATGATTCTCGCT464930.20625015193830692No Hit
GCCCGTCTAGCTCAGTTGGTAGAGC444630.1972447573964455No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT404660.17951344607436664No Hit
GCCCGTCTAGCTCAGTCGGTAGAGC397480.17632828682261467No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA356780.1582731361894245No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTG287980.12775239015592374No Hit
GAGAATTCCACGTTGTGGCCGTGGAGACCTGGGCT279230.12387075457753521No Hit
GTCAGGATAGCTCAGTTGGTAGAGC266130.11805939159731922No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATT233710.10367737726002131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)