FastQCFastQC Report
Fri 1 Sept 2023
SRR13808062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21300925
Total Bases576.1 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1449690.6805760782689014No Hit
GAGAATTCCACGTTGTGGCCGTGGAGACCTGGGCT791380.37152377185497815No Hit
CAGGATATTCGGCTCTCACCCGAAAGACCCGGGT552890.25956149791617034No Hit
GAGAATTCCACGTTGTGGCCGTGGAGACCTGGGC543080.2549560641145866No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTG387120.1817385864698364No Hit
GGGGCTATAGCTCAGCTGGTAGAGCGCTTGAAT353740.16606790550175637No Hit
GACGACTTGGCTCTTGTATCGATG254050.11926712102878162No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC235200.1104177400746681No Hit
GCCCAGGTGGCGGAATTGGTAGACGC227020.10657753125744539No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA224540.10541326256958325Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC217610.10215988272809749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)