FastQCFastQC Report
Fri 1 Sept 2023
SRR13808064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16814906
Total Bases459.2 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC5033922.993724734470713No Hit
GTGAAACCGTGCTGAGCTCCCAAGC2157291.28296286639961No Hit
GTCAGGATAGCTCAGTTGGTAGAGC1460300.8684556428682979No Hit
CGTGAAACCGTGCTGAGCTCCCAAGC1138180.6768875187289183No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT873770.5196401335814782No Hit
AGTGGGCCGGCGGGGCTTGTGCGAAAGCTC833130.4954711016523078No Hit
TGAAACCGTGCTGAGCTCCCAAGC794700.4726163797763723No Hit
GTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGA713260.42418316224901886No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTC710470.4225239201456137No Hit
GAAACCGTGCTGAGCTCCCAAGC700220.4164281382245015No Hit
CGTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGA689520.41006473660929177No Hit
TAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC599470.35651106226820417No Hit
AACCGTGCTGAGCTCCCAAGC552110.3283455762405095No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTG491800.29247859012711697No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGG475910.28302864137331485No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACT463320.2755412370428951No Hit
TCTTCGATGGTCTAGTGGTCATGATTTCCGCTT458310.2725617377819418No Hit
CGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA420510.2500816834777429No Hit
TGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGA418980.24917177651781106No Hit
AAACCGTGCTGAGCTCCCAAGC407010.24205309265481473No Hit
GCGAGCGTAGTTCAATGGTAAAAC322970.1920736280060085No Hit
AACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA282890.1682376339183817No Hit
AGGGCTATAGCTCAGTTCGGTAGAGC281280.1672801501239436No Hit
GCCCGTCTAGCTCAGTCGGTAGAGC261590.15557030173109504No Hit
GTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA258500.15373264649829144No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAGC250990.14926637115901809No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTC245680.14610845876866632No Hit
AACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGA239370.14235583594698656No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA220360.13105039064744103Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTAGTGGGCCGGCGGGGCTTGTGCGAAAGCTT215180.1279697906131619No Hit
GCCCCTATCGTCTAGTGGTCAGGACA209560.12462751798909848No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT203440.12098789014937103No Hit
AGCACATGACGAAGTGAGCGGGCTCT185360.11023552555096056No Hit
GTGGGCCGGCGGGGCTTGTGCGAAAGCTC184820.10991438191804344No Hit
CAGAGTTCTACAGTCCGACGATCCAG177510.10556704866503566Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAACGTGAAACCGTGCTGAGCTCCC177250.10541242395289037No Hit
ACCGTGCTGAGCTCCCAAGC171860.10220693472803238No Hit
TGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA168460.10018491926151714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)