FastQCFastQC Report
Fri 1 Sept 2023
SRR13808065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20063122
Total Bases556 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC5488122.7354267197298605No Hit
GTGAAACCGTGCTGAGCTCCCAAGC2333451.16305428437309No Hit
GTCAGGATAGCTCAGTTGGTAGAGC1782080.8882366363520093No Hit
CGTGAAACCGTGCTGAGCTCCCAAGC1475820.7355884094210263No Hit
CGTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGA1441860.7186618313939376No Hit
GTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGA1354840.6752887212668098No Hit
AGTGGGCCGGCGGGGCTTGTGCGAAAGCTC1203040.5996275155980211No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTC1053430.5250578648726754No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1024490.5106333899579537No Hit
TGAAACCGTGCTGAGCTCCCAAGC968960.4829557433783237No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTG847850.4225912597251813No Hit
TGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGA829720.41355477975960075No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGG718640.35818951806204435No Hit
TAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC663290.3306015883270809No Hit
GAAACCGTGCTGAGCTCCCAAGC660190.32905646489115703No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACT539850.2690757699624216No Hit
CGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA515710.2570437442388079No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTC513590.2559870791794019No Hit
AACCGTGCTGAGCTCCCAAGC509080.25373917379359007No Hit
GTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA504020.25121713360463044No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGC450640.22461110489185085No Hit
AACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA426400.21252923647675573No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA392700.19573224944751869Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGGCTATAGCTCAGTTCGGTAGAGC392360.19556278429648188No Hit
GCGAGCGTAGTTCAATGGTAAAAC386000.19239278911826385No Hit
AAACCGTGCTGAGCTCCCAAGC372790.18580856957356887No Hit
TGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA358900.17888541972680025No Hit
AACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGA332560.1657568547905954No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCC322170.16057819914567634No Hit
GCCCGTCTAGCTCAGTCGGTAGAGC298130.14859601611354403No Hit
GCCCCTATCGTCTAGTGGTCAGGACA288550.14382108626962445No Hit
GTAGTGGGCCGGCGGGGCTTGTGCGAAAGCTT267290.1332245300606755No Hit
AGCACATGACGAAGTGAGCGGGCTCT262370.13077226963978986No Hit
GTGGGCCGGCGGGGCTTGTGCGAAAGCTC248960.12408836471213204No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCC242710.12097319649454358No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT236780.11801752488969564No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAGC226510.11289868047455426No Hit
TTGTCGTCTAGTCCGGTCAGGATACCTGG218000.1086570674294858No Hit
GTGAAACCGTGCTGAGCTCCCAAGT202910.10113580528494019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)