FastQCFastQC Report
Fri 1 Sept 2023
SRR13808067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17417440
Total Bases467.7 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC3743322.1491792134779852No Hit
GTGAAACCGTGCTGAGCTCCCAAGC2646031.5191842199542527No Hit
CGTGAAACCGTGCTGAGCTCCCAAGC2449731.406481090217621No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTC2059551.1824642427360164No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT1603150.9204280307553807No Hit
TGAAACCGTGCTGAGCTCCCAAGC1405320.8068464711231962No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTG1240250.7120736457252042No Hit
AACCGTGCTGAGCTCCCAAGC1163070.6677617376606435No Hit
GAAACCGTGCTGAGCTCCCAAGC1093750.6279625478830413No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1049220.6023962189621437No Hit
AGTGGGCCGGCGGGGCTTGTGCGAAAGCTC995650.5716396898740572No Hit
AGAACGTGAAACCGTGCTGAGCTCCC903630.5188075859598196No Hit
AAACCGTGCTGAGCTCCCAAGC811550.4659410338143838No Hit
GCGAGCGTAGTTCAATGGTAAAAC755230.43360562746304854No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACT620050.3559937625736044No Hit
GACGACTTGGCTCTTGTATCGATG480110.27564900467577325No Hit
GGGGACGTAGCTCATATGGT448850.2577014762215343No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAGC445730.2559101681992302No Hit
TAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC398080.22855253125602845No Hit
ACCGTGCTGAGCTCCCAAGC394750.2266406544245308No Hit
ATGGACGACTTGGCTCTTGTATCG355370.20403113201480816No Hit
CGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA342660.19673384837266555No Hit
GCCCAGGTGGCGGAATTGGTAGACGC327980.1883055144728502No Hit
ATGGACGACTTGGCTCTTGTATCGATG308950.1773796838111686No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTC301780.173263120182989No Hit
AGCACATGACGAAGTGAGCGGGCTCT295840.1698527452943716No Hit
GTCAGGATAGCTCAGTTGGTAGAGC280140.1608387914641876No Hit
ACGACTTGGCTCTTGTATCGATG267810.1537596799529667No Hit
GCCCAGATGGCGGAATTGGTAGACGC239480.13749437345557097No Hit
CGTGAAACCGTGCTGAGCTCCCA235050.13495094571877383No Hit
GTGAAACCGTGCTGAGCTCCCAAGT223190.1281416786852718No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGG219810.12620109499444235No Hit
CGTGAAACCGTGCTGAGCTCCCAAGT205020.11770960600409704No Hit
GACGACTTGGCTCTTGTATCGATGAA199790.11470686851799117No Hit
GTGAAACCGTGCTGAGCTCCC194790.11183618258481154No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA191410.10989559889398214Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGTGAAACCGTGCTGAGCTCCC184880.10614648306524954No Hit
AGAACGTGAAACCGTGCTGAGCTCC178860.10269017720170127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)