FastQCFastQC Report
Fri 1 Sept 2023
SRR13808070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25256271
Total Bases685.1 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC3976771.574567361903901No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTC2168370.8585471703245503No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1232050.487819440961811No Hit
GTCAGGATAGCTCAGTTGGTAGAGC1201930.4758936899275431No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTG1068170.42293258573286613No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACT1050800.41605508588342277No Hit
GCGCCCGTAGCTCAACGGATAGAGCATCTGACT663450.26268723518210585No Hit
GTGAAACCGTGCTGAGCTCCCAAGC611310.24204285739569392No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGG596940.2363531813544446No Hit
AGTGGGCCGGCGGGGCTTGTGCGAAAGCTC548230.21706688212206784No Hit
TAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC535740.21212157566728676No Hit
GCCCGTCTAGCTCAGTCGGTAGAGC503790.1994712521100205No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTC493410.1953613817336692No Hit
AGCACATGACGAAGTGAGCGGGCTCT464950.18409289320660205No Hit
AGGGCTATAGCTCAGTTCGGTAGAGC428830.16979149455594614No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT398210.15766777288697922No Hit
TTGTCGTCTAGTCCGGTTAGGATACCTGG328840.1302013270288397No Hit
GCGAGCGTAGTTCAATGGTAAAAC322720.1277781664601239No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCC313730.12421865444823585No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCC313200.1240088055754549No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA291360.1153614482518025Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGTGAAACCGTGCTGAGCTCCCAAGC287450.11381331788845629No Hit
TTGTCGTCTAGTCCGGTCAGGATACCTGG274090.10852354252929895No Hit
TTGTCGTCCAGCGGTTAGGATATCTGG273360.10823450540263843No Hit
GGGTGTTTGGTCTAGTGGTATGATTCTCGCT255710.10124614199776365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)