FastQCFastQC Report
Fri 1 Sept 2023
SRR13808074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15525938
Total Bases412 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT861650.5549745207020664No Hit
GTAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC607270.39113256796465373No Hit
AGTGGGCCGGCGGGGCTTGTGCGAAAGCTC603740.3888589533205659No Hit
TAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC310530.20000723949818683No Hit
AACCGTGCTGAGCTCCCAAGC276280.17794738069931748No Hit
AGCACATGACGAAGTGAGCGGGCTCT203120.1308262341379954No Hit
ACCGTGCTGAGCTCCCAAGC203090.130806911633938No Hit
CGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA196760.1267298632778258No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA186080.11985105183339004Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTGAAACCGTGCTGAGCTCCCAAGC176090.11341665798227456No Hit
GTGGGCCGGCGGGGCTTGTGCGAAAGCTC169700.10930096461804754No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT167300.10775516429345526No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTG166420.10718837084110473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)