FastQCFastQC Report
Fri 1 Sept 2023
SRR13808075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27539481
Total Bases741.7 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC14337855.206289109079433No Hit
AGTGGGCCGGCGGGGCTTGTGCGAAAGCTC4078181.480848531604499No Hit
TAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC2335980.848229492778023No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1070610.38875460289175384No Hit
GTGGGCCGGCGGGGCTTGTGCGAAAGCTC664300.24121732722559297No Hit
GTAGTGGGCCGGCGGGGCTTGTGCGAAAGCTT538080.19538494570758252No Hit
GTGAAACCGTGCTGAGCTCCCAAGC505990.18373258377672405No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTC500410.18170640180183498No Hit
CGTGAAACCGTGCTGAGCTCCCAAGC400760.14552198714274972No Hit
GACGACTTGGCTCTTGTATCGATG371390.13485729814588734No Hit
ATGGACGACTTGGCTCTTGTATCGATG318640.11570297929724964No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA295140.1071697756395627Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACCGTGCTGAGCTCCCAAGC292970.1063818159826614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)