FastQCFastQC Report
Fri 1 Sept 2023
SRR13808077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7789389
Total Bases206.5 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGCTGGTTAGGAT720290.9247066746826997No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT440610.565654122550562No Hit
AGTGGGCCGGCGGGGCTTGTGCGAAAGCTC395650.5079345761265742No Hit
ATAGCTCAGTCGGTAGAGCGACGG248600.31915211834971907No Hit
GCCCAGGTGGCGGAATTGGTAGACGC240770.3090999820396696No Hit
GCCCAGATGGCGGAATTGGTAGACGC228560.293424811625148No Hit
CGGAGTGTGGCTCAGTCTGGTAGAGC210490.27022658644984865No Hit
GGGGCTATAGCTCAGCTGGTAGAGCGCTTGAAT189950.24385738085490402No Hit
TAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC154140.1978845837587518No Hit
AGGAGTGTAGCTCAGCTGGTAGAGC118500.1521300322785266No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA115320.14804755546295095Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTGGGCCGGCGGGGCTTGTGCGAAAGCTC112890.14492792695293558No Hit
GCGGGCGTAGCTCAGGGGTAGAGC111930.1436954811218184No Hit
GTGAAACCGTGCTGAGCTCCCAAGC108260.1389839434132767No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTC95150.12215335503208274No Hit
GTAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC94350.1211263168394851No Hit
CGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA91120.11697965013687209No Hit
GCCCAGGTGGCGGAATTGGTAGACG86130.11057349941054426No Hit
ACGCTTAACTGCGGAGTTCTGATGGGATC80910.10387207520384462No Hit
GGTGTCGTGGTGTAGTTGGTTATC79160.10162542915753726No Hit

[OK]Adapter Content

Adapter graph