FastQCFastQC Report
Fri 1 Sept 2023
SRR13808078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22222637
Total Bases586.3 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1035270.4658628046707508No Hit
AGTGGGCCGGCGGGGCTTGTGCGAAAGCTC607890.27354539427521585No Hit
TAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC588630.2648785560417515No Hit
GTAGTGGGCCGGCGGGGCTTGTGCGAAAGCTC494130.22235434975606178No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT379670.170848311116273No Hit
GGGGCTATAGCTCAGCTGGTAGAGCGCTTGAAT257560.11589983672954744No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCT250590.11276339527122727No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA232530.10463654695885101Illumina DpnII expression Sequencing Primer (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)