FastQCFastQC Report
Fri 1 Sept 2023
SRR13808079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8425876
Total Bases224.4 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCTGTAGTTTAGTGGTAAGAATTCC2927743.4747010281186195No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATTCC532940.6325039675399923No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT347010.41183848421220537No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACG297220.35274670550575393No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA246160.29214766512111023Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTCT241360.28645092806967487No Hit
ATTTATTAGACTGATGATTCATGATAACT236350.28050495877223924No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATCCC208360.24728586084105678No Hit
AGAGTTCTACAGTCCGACGATC205790.24423573287810077Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGAGTTCTACAGTCCGACGATCCAG185380.22001273220730996Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTC181010.21482632785006567No Hit
CAGAGTTCTACAGTCCGACGATC178710.21209664134625292Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATCAG135290.16056490743514384Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGTGGCTGTAGTTTAGTGGTAAGAATT109440.12988560477272632No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCA104850.12443809996729124No Hit
AGGGCTATAGCTCAGTTAGGTAGAGCACCTCGT101650.12064027526633432No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCAC99260.11780377494280712No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGA97040.11516903405651828Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC91790.10893822790651085No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCA91480.10857031363860564No Hit
GAGTTCTACAGTCCGACGATC87900.1043214972544101Illumina DpnII expression Sequencing Primer (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)