FastQCFastQC Report
Fri 1 Sept 2023
SRR13808080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8062182
Total Bases220.9 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCTGTAGTTTAGTGGTAAGAATTCC3065723.8025933922106945No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACG1376001.7067339834302924No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1141941.4164155559872005No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA945191.1723749228186613Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTGGCTGTAGTTTAGTGGTTAGAATTCC583720.7240223552383214No Hit
CAGAGTTCTACAGTCCGACGATCCAG511490.6344312246982269Illumina DpnII expression Sequencing Primer (100% over 23bp)
ATTTATTAGACTGATGATTCATGATAACT394920.4898425761164905No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATCCC365640.45352486460861335No Hit
AGAGTTCTACAGTCCGACGATCAG352580.4373257760740206Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAGTTCTACAGTCCGACGATC341990.4241903742684052Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGA340150.4219081137091671Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGAGTTCTACAGTCCGACGATC293910.3645539135683119Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTCT275950.3422770659357479No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCAC241280.2992738194201024No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC202740.25147038357605916No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATCCCACG202610.251309136906113No Hit
AGGGCTATAGCTCAGTTAGGTAGAGCACCTCGT191610.23766518791066737No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATTCCACG170480.21145640224941586No Hit
TCCGTCCAAGGCTAAATACGTACCCGCTG169080.2097198996499955No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTC161770.20065287536302207No Hit
TCAGAGTTCTACAGTCCGACGATCTCAG146410.1816009611293816Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGAGTTCTACAGTCCGACGATCAGAGTTCTAC138900.17228586504249097Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC134600.16695232134427132No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCA133210.16522822233484682No Hit
GAGTTCTACAGTCCGACGATC130470.16182963867598127Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTGGCTGTAGTTTAGTGGTAAGAATT129290.16036601505646983No Hit
CCGTCCAAGGCTAAATACGTACCCGCT128090.15887758425696666No Hit
GAGAATTTCTGGAAGGGCTCCCTTCGGGGTAAAGCCCTA118940.14752829941075504No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCA112730.1398256700233262No Hit
TCATAGTCAAAAGAAGAGTTTGATCCTGGC111080.13777907767400935No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACGT98020.1215799891394166No Hit
CAGAGTTCTACAGTCCGACGATCCAGAGTTCTAC97500.1209350024596319Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAATTTCTGGAAGGGCTCCCTTCGGGGTAAAGCCCTA96060.1191488855002281No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACA95570.11854110959043099No Hit
GAGTTCTACAGTCCGAGATCGAGTTCTACAGTCCG90020.11165711714272886No Hit
CCGTCCAAGGCTAAATACGTACCCGCTG83330.10335911543549872No Hit
CCGTCCAAGGCTAAATACGTACCCGCTGAG82100.10183347386600798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)