FastQCFastQC Report
Fri 1 Sept 2023
SRR13808083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7082278
Total Bases207.9 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3034374.284454803948673No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA2934574.1435396916077005Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGAGTTCTACAGTCCGACGATCCAG1495572.1117075607594056Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC1307981.8468351567108774Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGA1184261.6721456006104252Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGAGTTCTACAGTCCGACGATC1181141.6677402383809277Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATCAG1071661.5131572073279247Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACG943621.3323679189097068No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACGTT866861.2239847122634837No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCC767621.0838603059637026No Hit
GAGTTCTACAGTCCGACGATC525730.7423176554210382Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTTCTACAGTCCGACGATC507980.7172550978654043No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC498510.703883693918821No Hit
AGGGCTATAGCTCAGTTAGGTAGAGCACCTCGT473770.6689514305990247No Hit
TCAGAGTTCTACAGTCCGACGATCTCAG416950.5887230069195251Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGAGTTCTACAGTCCGACGATCAGAGTTCTAC411880.5815642932965919Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACGT376290.5313121004287039No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACGTTG345600.4879785854212444No Hit
GGCGGATGTAGCCAAGTGGATTAAGGCAGTG299710.423183049295721No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATCCCACGT275310.38873085750093406No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATT270440.3818545388927122No Hit
CAGAGTTCTACAGTCCGACGATCCAGAGTTCTAC265720.3751900165455239Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCG246970.3487154839163331Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGGCTATAGCTCAGTTAGGTAGAGCA240350.33936820892938685No Hit
GGCGGATGTAGCCAAGTGGATTAAGGCAGTGGATT238280.3364454205271242No Hit
GAGTTCTACAGTCCGACGATCG220700.3116228987339949Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTC218990.3092084213582127No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATCCC205140.28965256658945043No Hit
GGCGGATGTAGCCAAGTGGATTAAGGCAGTGGATTGTG198930.28088420138266246No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATCCCACGTTG190290.2686847367471314No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATCCCACGTT184920.2611024306021311No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCA179240.25308241218432825No Hit
CAGAGTTCTACAGTCCGACGATCC172910.24414460996871348Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTCCAC164660.2324958156118695No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA160650.22683379556690655No Hit
TTCAGAGTTCTACAGTCCGACGATCTTCAG157440.22230135558078912Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCG152370.21514264195785596Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCAAACTTGAGAGTTTGATCCTGGCTCAGAACG146270.20652959400915918No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTG146060.20623307924371226No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATCCCACG138180.19510671566408433No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT137220.1937512195934698No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG123130.1738564908070539No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATTCCACGTT117810.1663447834157315No Hit
GGTGGCTGTAGTTTAGTGGTAAGA117600.16604826865028455No Hit
GGCGGATGTAGCCAAGTGGATTAAGGCAGTGG116520.16452333557084317No Hit
AGGGCTATAGCTCAGTTAGGTAGAGCACCTCG114220.16127579290166244No Hit
AGGGCTATAGCTCAGTTAGGTAGAGCACCTCGTT113870.16078160162591754No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC108590.15332637323753742No Hit
AGGGCTATAGCTCAGTTAGGTAGAGCAC107890.15233799068604764No Hit
GGTGGCTGTAGCTCAGTTGGTAGAGTC106660.15060126134557272No Hit
AATATTTCTCAATGGACGTCTTGGC106510.15038946508453918No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATTCC104250.1471984014183007No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTAC97240.13730045615266726Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACGTTGTG84270.11898713944863504No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATTCCACG83260.11756104462434262No Hit
AGGGCTATAGCTCAGTTAGGTAGAGCACC75470.10656175880133484No Hit
AGAGTTCTACAGTCCGACGATT75080.10601108852264765Illumina DpnII expression Sequencing Primer (95% over 21bp)
CAGAGTTCTACAGTCCGACGATT73220.10338481488583193Illumina DpnII expression Sequencing Primer (95% over 22bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTCT72640.10256586934316897No Hit
TCAGAGTTCTACAGTCCGACGATCTCAGAGTTCTAC71490.1009420980085786Illumina DpnII expression Sequencing Primer (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)