FastQCFastQC Report
Fri 1 Sept 2023
SRR13808084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4513737
Total Bases121.8 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCTGTAGTTTAGTGGTAAGAATTCC623861.3821363539789757No Hit
AGAGTTCTACAGTCCGACGATC278470.6169389133660201Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGAGTTCTACAGTCCGACGATC224720.4978579833074014Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCATTTATTAGACTGATGATTCATGATAACTC216280.47915950796424334No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACG209540.4642273131996835No Hit
ACAGAGTTCTACAGTCCGACGATC191510.424282584474904Illumina DpnII expression Sequencing Primer (95% over 24bp)
TCATAGTCAAAAGAAGAGTTTGATCCTGGC181110.40124180917053875No Hit
AGTTCTACAGTCCGACGATC132510.2935704938059085No Hit
AAGAGTTCTACAGTCCGACGATC127480.28242673421158565Illumina DpnII expression Sequencing Primer (95% over 23bp)
GAGTTCTACAGTCCGACGATC114930.2546227217048756Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCATTTATTAGACTGATGATTCATGATAACT114350.25333775538982445No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATTCC113150.2506792043931669No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACGT111230.24642552279851485No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT86920.1925677105245609No Hit
GGGAGACCATCGACTACGCCTTTCGGCCTGATC86820.19234616460817278No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATCCC83730.18550039579177963No Hit
ATTTATTAGACTGATGATTCATGATAACT80370.1780564530011385No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTC66890.14819206347201885No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATT62010.13738062275227822No Hit
GGGAGACCATCGACTACGCCTTTCGGCCTGA58540.12969297945361016No Hit
GAAAAGGACTTTGAAAAAAAAAAAA58330.1292277330291951No Hit
GAAAAGGACTTTGAAAAAAAAAAA55140.12216041829641383No Hit
GAGAATTTCTGGAAGGGCTCCCTTCGGGGTAAAGCCCTA54980.1218059448301928No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACGTT51820.11480509387232796No Hit
GAAAAGGACTTTGAAAAAAAAAAAAA50840.1126339438917243No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATCCCACGT48700.10789286128101837No Hit
AAGTTCTACAGTCCGACGATC48240.10687375006563297Illumina DpnII expression Sequencing Primer (95% over 21bp)
TCCGTCCAAGGCTAAATACGTACCCGCTG45430.10064830981512658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)