FastQCFastQC Report
Fri 1 Sept 2023
SRR13808085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998029
Total Bases247.2 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2168002.409416551113583No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA1146451.2741123639410366Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGAGTTCTACAGTCCGACGATCCAG839140.9325820132386771Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATCAG725120.8058653734056648Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTCC602210.6692687920876894No Hit
AGAGTTCTACAGTCCGACGATC579550.6440854991687623Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAAT487600.541896453101007No Hit
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGA486070.5401960807194554No Hit
CAGAGTTCTACAGTCCGACGATC472130.5247037990208745Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAATT385850.42881613295533944No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGA378300.4204254064973562Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAA373880.4155132196173184No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTG360480.4006210693475204No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC357920.3977760018332904No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACG265150.2946756450773831No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTC264370.29380878856914106No Hit
TCAGAGTTCTACAGTCCGACGATCTCAG249390.2771606981929043Illumina DpnII expression Sequencing Primer (100% over 24bp)
GAGTTCTACAGTCCGACGATC244210.27140388189457937Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTTCTACAGTCCGACGATC230390.256044962735728No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT229200.25472245088341017No Hit
GACGACTTGGCTCTTGTATCGATG212990.23670739447494557No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATT212120.23574051606190644No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTAC200090.22237092145402068Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGTTCTACAGTCCGAGATCGAGTTCTACAGTCCG191660.2130022030380209No Hit
GTGTTATCGCAAGGCATTTACGTATG183630.20407802642111958No Hit
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAATTT182970.20334453245260714No Hit
GGTGGCTGTAGTTTAGTGGTAAGA175980.19557616451336174No Hit
ATGGACGACTTGGCTCTTGTATCGATG175340.19486489763480425No Hit
GTGTTATCGCAAGGCATTTACGTATGAGTG171200.19026389001413532No Hit
AGGGCTATAGCTCAGTTAGGTAGAGCACCTCGT168030.18674089625628013No Hit
TTTAAACGTGTGTTTTCTGAAG158270.17589407635827803No Hit
GTGTTATCGCAAGGCATTTACGTATGAGT157890.1754717616491345No Hit
ATGGACGACTTGGCTCTTGTATCG151800.16870361275786064No Hit
TCAAACTTGAGAGTTTGATCCTGGCTCAGAACG143980.160012820585486No Hit
GTGTTATCGCAAGGCATTTACGTATGAGTGTCAGC135070.15011065201056809No Hit
AATATTTCTCAATGGACGTCTTGGC129960.14443163052708544No Hit
GAGTTCTACAGTCCGACGATCG129770.14422047317251369Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACGACTTGGCTCTTGTATCGATGAAG127050.14119758893864423No Hit
GATTTCTTGTCGATTGGTTGAAG126580.14067525232470357No Hit
CAGAGTTCTACAGTCCGACGATCCAGAGTTCTAC126190.14024182407058255Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGGCTATAGCTCAGTTAGGTAGAGCA122670.13632985623851623No Hit
GACGACTTGGCTCTTGTATCGATGAA120100.13347367517930872No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCG116390.1293505499926706Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTGGCTGTAGTTTAGTGGTTAGAATTCC110570.12288246681578822No Hit
CGCGACCTCAGGTCAGACGTGGCGACCC105940.1177368954912237No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA103260.1147584654372641No Hit
GAACCGTGGGTGCTGAATACC102760.11420278818839104No Hit
ATACAGGAACCGTGGGTGCTGAATACC102650.11408053919363895No Hit
ACGACTTGGCTCTTGTATCGATG100670.11188005728810166No Hit
GACGACTTGGCTCTTGTATCG99020.11004632236682056No Hit
GGTGGCTGTAGCTCAGTTGGTAGAG98430.10939062321315034No Hit
TTCAGAGTTCTACAGTCCGACGATCTTCAG96940.10773470501150863Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGAGTTCTACAGTCCGACGATCC96850.10763468310671148Illumina DpnII expression Sequencing Primer (100% over 23bp)
ATGGACGACTTGGCTCTTGTATCGATGAA94800.10535640638633195No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG93980.10444509569818013No Hit
TGGACGACTTGGCTCTTGTATCG93170.10354489855500576No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC91110.10125550828964877No Hit
ATGGACGACTTGGCTCTTGTATCGATGAAG90670.10076651231064046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)