FastQCFastQC Report
Fri 1 Sept 2023
SRR13808086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10792666
Total Bases319.2 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT7924297.342291515367936No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTG2123191.9672525768887874No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT2065161.9134845829566114No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA1830711.696253733785517Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC1494471.3847088383908108No Hit
CAGAGTTCTACAGTCCGACGATCCAG1025740.9504046544199553Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGA884880.8198900994434554Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAGTTCTACAGTCCGACGATCAG622260.5765581923873119Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTCC466130.4318951406445822No Hit
AGAGTTCTACAGTCCGACGATC451730.41855274683752836Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT400290.37089075118233067No Hit
CAGAGTTCTACAGTCCGACGATC398890.3695935740066449Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA397970.36874114329119423No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTAC382730.354620443178729Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTG378430.35063625613912264No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACG373490.34605907381920276No Hit
TCAGAGTTCTACAGTCCGACGATCTCAG356630.33043735440344396Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC331530.30718082075364883No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTC263530.24417507222033924No Hit
CAGAGTTCTACAGTCCGACGATCCAGAGTTCTAC258190.2392272678502235Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTGTTATCGCAAGGCATTTACGTATGAGTGTCAGC237730.22026994998270122No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTT215040.19924641418533662No Hit
GAGTTCTACAGTCCGAGATCGAGTTCTACAGTCCG205170.19010131509675182No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTA187820.17402558366950296No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT184510.17095868620413157No Hit
TCAAACTTGAGAGTTTGATCCTGGCTCAGAACG178200.16511212336229067No Hit
AGGGCTATAGCTCAGTTAGGTAGAGCACCTCGT170810.1582648809849207No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT168190.15583730655613728No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTG163700.15167707404268788No Hit
TTCAGAGTTCTACAGTCCGACGATCTTCAG160900.1490827196913163Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGTTCTACAGTCCGACGATC159630.1479059946819442Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCG159540.14782260472065012Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAATTT157700.1461177432897488No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG156840.14532090588182753No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCT155350.14394033874484766No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCG153880.1425783027103776No Hit
AGTTCTACAGTCCGACGATC153400.14213355625014246No Hit
GGAATACCGGGTGCTGTAGGCTT147090.13628699340830153No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATT146930.13613874458822314No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT145710.13500834733512554No Hit
TAGATATAGGAATAGAAAACTCT143390.1328587394439891No Hit
AAACCGTTACCATTACTGAGT143220.13270122507265583No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTC136060.12606709037414854No Hit
AATATTTCTCAATGGACGTCTTGGC133560.1237507025604239No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATCCC129610.120090809814739No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTT125890.11664402474791677No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT122480.1134844717699964No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGT120440.1115942993139971No Hit
GGGAGACCATCGACTACGCCTTTCGGCCTGATC120320.11148311269893833No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCG111610.10341281755592178Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGAATACCGGGTGCTGTAGGCTTT109080.10106863308843245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)