FastQCFastQC Report
Fri 1 Sept 2023
SRR13808087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8870226
Total Bases246.5 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2187512.4661265676883546No Hit
AGCTGGTCCGCGGGCTGTGGGTT512590.5778770461992738No Hit
GACGACTTGGCTCTTGTATCGATG492760.5555213587568119No Hit
ATGGACGACTTGGCTCTTGTATCGATG434880.4902693572858234No Hit
TCAGTGCACTACAGAACTTTGT382350.43104876921963425No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC333630.37612344939125564No Hit
GACGACTTGGCTCTTGTATCGATGAAG309860.34932593600208156No Hit
GGCTGGTCCGATGGTAGTGGGTT306700.34576345630877947No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT300270.33851448655310473No Hit
CAGAGTTCTACAGTCCGACGATCCAG289360.3262149126752802Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACGACTTGGCTCTTGTATCGATGAA280260.3159558730521635No Hit
ATGGACGACTTGGCTCTTGTATCG280200.3158882310326704No Hit
GATTTCTTGTCGATTGGTTGAAG260200.2933408912016447No Hit
ATGGACGACTTGGCTCTTGTATCGATGAAG260090.2932168808325741No Hit
GGTGGCTGTAGCTCAGTTGGTAGAGTC259110.2921120611808538No Hit
TCAAACTTGAGAGTTTGATCCTGGCTCAGAACG253630.28593409006715276No Hit
ATGGACGACTTGGCTCTTGTATCGATGAA247030.2784934679229143No Hit
AGACGATACAGGAACCGTGGGTGCTGAATACC226370.2552020658774647No Hit
TTTAAACGTGTGTTTTCTGAAG220690.2487986213654534No Hit
ACGACTTGGCTCTTGTATCGATG216010.24352254384499336No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTG212130.23914835991777436No Hit
AGAGTTCTACAGTCCGACGATCAG197970.22318484331740815Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATC189890.21407571802567377No Hit
TTGGAAGCGAATTAAGGGCGTATG188550.21256504625699504No Hit
TGGACGACTTGGCTCTTGTATCGATG180680.2036926680334864No Hit
GACGACTTGGCTCTTGTATCGATGA170940.19271211353577689No Hit
TGGACGACTTGGCTCTTGTATCG162330.1830054837385203No Hit
GACGACTTGGCTCTTGTATCG159680.1800179612109094No Hit
ACGACTTGGCTCTTGTATCGATGAAG158100.17823672136425836No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA156450.17637656582819874Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC153750.17333267495101026No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTC153070.1725660653967554No Hit
ACGACTTGGCTCTTGTATCGATGAA152780.1722391289692055No Hit
ATGGACGACTTGGCTCTTGTATCGATGA140510.15840633598287124No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCC134560.1516985023831411No Hit
GACGATACAGGAACCGTGGGTGCTGAATACC133990.15105590319795686No Hit
GTGTTATCGCAAGGCATTTACGTATG133000.14993980987632108No Hit
GTGTTATCGCAAGGCATTTACGTATGAGT131510.14826003305890967No Hit
GTTTAAACGTGTGTTTTCTGAAG131210.14792182296144427No Hit
GAACCGTGGGTGCTGAATACC129950.14650134055208966No Hit
ATACAGGAACCGTGGGTGCTGAATACC127300.14351381802447874No Hit
GTGTTATCGCAAGGCATTTACGTATGAGTG122990.1386548662908927No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTC105080.11846372347220917No Hit
GTGTTATCGCAAGGCATTTACGTATGAGTGTCAGC105040.11841862879254711No Hit
TTCAAGTAATCCAGGATAGGCT103220.11636682086792378No Hit
AAACCGTTACCATTACTGAGT102300.11532964323569658No Hit
TGGACGACTTGGCTCTTGTATCGATGAAG102040.11503652781789325No Hit
AACCGTGGGTGCTGAATACC101190.11407826587507466No Hit
CCGTGTGAAAGTAGGACATCGTCAGGCT98650.11121475371653439No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA98450.11098928031822414No Hit
TCAGAGTTCTACAGTCCGACGATCTCAG97390.10979427130717978Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGGACGACTTGGCTCTTGTATCGATGAA96610.10891492505376978No Hit
GAGACGATACAGGAACCGTGGGTGCTGAATACC91970.10368394221297181No Hit
GGTGGCTGTAGCTCAGTTGGTAGAGT91860.10355993184390117No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGT91710.10339082679516848No Hit
ATTTCTTGTCGATTGGTTGAAG91620.10328936376592884No Hit
AATGGACGACTTGGCTCTTGTATCGATG88870.10018910453916281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)