FastQCFastQC Report
Fri 1 Sept 2023
SRR13808088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13808088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3897563
Total Bases123.5 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT43080611.05321453431285No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC547481.4046726120911965No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT440521.130244719585033No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATT438941.1261909044189922No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTA302430.775946405484658No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA257330.660233073846401Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA242870.6231329679597225No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACT178090.4569265461520442No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCC168810.433116796316057No Hit
TCCGTCCAAGGCTAAATACGTACCCGCTG161070.41325823341405904No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACG158820.40748539536115264No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG155440.39881330975278656No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGA144520.37079580240268084Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGAATTTCTGGAAGGGCTCCCTTCGGGGTAAAGCCCTA127160.3262551496922564No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC126390.3242795562252618No Hit
TCCGTCCAAGGCTAAATACGTACCCGCTGAG119610.30688407089250386No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT115650.29672387591938865No Hit
GAGAATTTCTGGAAGGGCTCCCTTCGGGGTAAAGCCCT101910.2614710782096402No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAA97140.24923266153747867No Hit
CCGTCCAAGGCTAAATACGTACCCGCT88300.22655182225405976No Hit
CCGTCCAAGGCTAAATACGTACCCGCTGAG83130.21328712326138155No Hit
TTCCGTCCAAGGCTAAATACGTACCCGCTG78990.20266510124403378No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAC75480.1936594738814998No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTT71360.18308876598017787No Hit
CCGTCCAAGGCTAAATACGTACCCGCTG69700.1788296943500336No Hit
GGGAGACCATCGACTACGCCTTTCGGCCTGATC68570.17593044679457395No Hit
TTCCGTCCAAGGCTAAATACGTACCCGCTGAG68260.1751350779961735No Hit
AGAGTTCTACAGTCCGACGATCACTTTAAATAGGTAGGACGTGTCGGCT66840.17149177575833927Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTCAAGTAATCCAGGATAGGCT64830.16633470709774287No Hit
GCATTTATTAGACTGATGATTCATGATAACTC63260.16230654898971486No Hit
TCCGTCCAAGGCTAAATACGTACCCGCTGA59790.15340354985923255No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTC58750.15073521582588914No Hit
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAATTT58350.14970893350537245No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCGGC56010.1437051819303498No Hit
GAGTTCTACAGTCCGACGATCACTTTAAATAGGTAGGACGTGTCGGCT54290.13929216795212804Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATT54250.13918953972007636No Hit
GCATTTATTAGACTGATGATTCATGATAACT50310.12908065886298695No Hit
GGGAGACCATCGACTACGCCTTTCGGCCTGA50240.1289010594568965No Hit
TCCGTCCAAGGCTAAATACGTACCCGCT49900.12802871948445735No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT49140.12607878307547563No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCCACGTT45980.11797115274339376No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT43490.11158254529817734No Hit
ATTCCGTCCAAGGCTAAATACGTACCCGCTG42460.10893986832284687No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTC41490.10645113369559388No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATT41390.10619456311546471No Hit
GAGTTCTACAGTCCGACGATCACTTTAAATAGGTAGGACGTGTCGGCTG40120.10293611674782421Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTGGCTGTAGTTTAGTGGTAAGAATTCCA39870.10229469029750127No Hit
AGAGTTCTACAGTCCGACGATCACTTTAAATAGGTAGGACGTGTCGGCTG39790.10208943383339794Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAATTTCTGGAAGGGCTCCCTTCGGGGTAAAGCCCTA39180.10052435329460999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)