FastQCFastQC Report
Wed 28 Jun 2023
SRR1382093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1382093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50509297
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length40-51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA12092532.394119640984114No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA4850110.9602410423570139No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAAAAAAAAA4797520.9498290977995596No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAAAAAAAAA3671430.7268820233233497No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA3614410.7155930125101523No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA3582980.7093703957115063No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAAAAAAAA2921470.5784024275768479No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAA2747910.5440404367536535No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAAAAAA2378000.47080441448234767No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAA2275340.45047944341810975No Hit
GGGGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAAA2203320.4362206823032995No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAAAAA1963260.3886927984762884No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1791640.3547148953587693No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAAAAAAAA1777590.3519332292429253No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAA1626570.3220337832062878No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA1613270.3194006046055244No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA1605500.317862273949289No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAAAAAAAAAAA1507170.2983945708054499No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAA1495640.2961118227402769No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAA1475930.2922095708439577No Hit
GGGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAAAA1396100.2764045597387744No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAAAAAAAA1384880.27418318651316803No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA1325400.26240713665050613No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAAAAA1324610.2622507298012879No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAAAAAAAAA1277740.2529712500255151No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAAAA1133820.2244774857983076No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAAAAAAA1077140.21325578932528005No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAAAAAAAAA1026780.2032853476459987No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAA1015100.20097290207780955No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAAAAAAAAAAAA1000340.1980506677810226No Hit
GGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAA976090.19324957146008187No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAA973150.19266750040096578No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAAAAAAAA921480.18243770052867694No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAA918290.18180613363120063No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCAAAAAAAAA838780.1660644771991184No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA831840.1646904727262389No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAAAAAA820930.16253047434019918No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAAAAAAA809990.16036453645355628No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAAAAAA807510.15987353773702295No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA807010.1597745460603025No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA806420.15965773588177243No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCAAAAAAAAAA681550.13493555453761313No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAAA673900.13342098188379062No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA628610.1244543158064544No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA609050.12058176141315133No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAA575700.11397901657589889No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA572170.11328013533825267No Hit
GGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAAAAA564290.11172002651313875No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAAA536220.10616263378205401No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAA535640.10604780343705833No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAAAA525930.10412538507514764No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAAAAAAA520030.10295728328984662No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAAA509310.10083490174096067No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAA508150.1006052410509693No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)