FastQCFastQC Report
Wed 28 Jun 2023
SRR1382094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1382094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134834145
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length40-51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA21174771.5704308430182876No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA10593630.7856785831215083No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA9238750.685193650317581No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAAAAAAAAA7835080.5810901978871894No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAA6868960.5094377243983711No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAAAAAAAAA6193360.4593317219462474No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA5725810.4246557873007612No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAAAAAA5415960.4016757031388451No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAA5078670.3766605261597498No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAAAAA5046310.3742605406071289No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAAAAAAA4959380.3678133606290899No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAAAAAAAA4934220.3659473644454081No Hit
GGGGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAAA4396850.3260932162250148No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA4315450.3200561697483972No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAAAAAAAA4163330.308774161025755No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAA3649650.27067698615955177No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAAAAAAAAA3524400.2613877960957145No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA3458300.25648547702809255No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAAAAAAAA3351000.2485275521270966No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAA3326040.24667638898144087No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAAAA3145390.23327844738437728No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAAAAAAAAAAA3144140.23318574089671423No Hit
GGGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAAAA3082950.2286475729126328No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAAAAA2943160.21828002098429888No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA2735140.20285217813336526No Hit
GGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAA2638930.19571674519091586No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCAAAAAAAAAA2481850.1840668771252267No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAAAAAAA2408130.17859941930881085No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAAAAAAA2402680.17819521902259994No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA2360480.17506544799909549No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA2137420.15852216068860006No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAAAAAAAA2089530.1549703897332534No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAAAAAAAAA2083480.15452169033296426No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAAA2054180.15234865026214242No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAAAAAAAAAAAA2023360.15006287910232236No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA1941460.1439887500306395No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAAAAAAAAA1934340.14346069387691077No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAAAAAA1891640.1402938402583411No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAA1874510.1390233905514067No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAA1777840.13185384162149727No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA1752580.12998042891880243No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAA1539990.11421365114897268No Hit
TTGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAAA1488530.11039711046486037No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAAA1470460.10905694547920337No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAAA1469010.10894940595351424No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCAAAAAAAAA1450260.10755880863856852No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA1414020.10487106214824146No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCAAAAAAAAAA1410840.10463521684362666No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAAAAAAAAAA1361360.1009655232359726No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)