FastQCFastQC Report
Wed 28 Jun 2023
SRR1382096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1382096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79904383
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA17770212.223934324103347No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA17030872.1314062333727053No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA6884710.8616185672818473No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAA6822810.8538718082586284No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA6272250.7849694553050988No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA5382840.6736601670524132No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA5246120.6565497164279461No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAAAAAAAAA3738710.46789798752341283No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA3502220.4383013632681451No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAA3006540.3762672192838283No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA2691400.3368275805345997No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA2135620.26727194677168087No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAA2119590.26526579899878583No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAA1989960.24904265889894925No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAAAAAAAAAAAA1949160.2439365560209632No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAAAAAAAAA1933590.2419879770550259No Hit
TTGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAA1900000.2377842026513114No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAAAAAAAAA1866350.2335729192727763No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAA1822720.22811264308242016No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAA1796580.2248412330522595No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA1785230.22342078531536877No Hit
TTGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA1769740.22148221831585885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1752590.2193359030129799No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAAAAAAAA1718320.2150470268946323No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAA1701730.21297079535674532No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA1677090.20988710969709884No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAA1619420.20266973339872985No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAAAAAAAAAAA1552080.194242160658446No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAA1528430.19128237308333887No Hit
TTGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAAA1509190.18887449515754348No Hit
TGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAAAA1404500.17577258559145623No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA1403490.17564618451531No Hit
TGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA1352130.16921850206890401No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1346510.16851516142737752No Hit
TTGCGTGCATTTATCAGATCAAAACCAACAAAAAAAAAAA1267790.15866338646279265No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA1262110.15795253684644558No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA1221120.1528226555481944No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCAAAAAAAAAA1187820.14865517452277932No Hit
TTGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA1127620.14112116978614303No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAA1095340.13708134133267758No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAAAAA1075040.13454080485171882No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAA1058200.13243328591874615No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAAAAAAAA1017880.12738725483932464No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAA1007480.1260856992037596No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAAA1000560.12521966410778743No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA991730.12411459331336054No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAA965650.12085069225802043No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA931780.11661187597180994No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCAAAAAAAAA927900.1161262956000799No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA924740.11573082292619667No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAA896580.11220661074374357No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA895840.1121140000542899No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA888140.11115034828565037No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAAAAAA863640.10808418356725187No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAA849910.1063658798291453No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAAAAAAAA835450.10455621689738846No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA817200.10227223705613245No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCACGAAAAAAAAAAAA800980.10024231086297232No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAA800650.10020101150145919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)