FastQCFastQC Report
Wed 28 Jun 2023
SRR1382098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1382098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152099640
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA73127124.807843069188066No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA64840984.263059399746114No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA35830262.3557097176561363No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA23050441.5154828768825488No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA20620821.355744168756744No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA18448281.2129075387686652No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA16668631.0959020021349164No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAA13536410.889969890790011No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA12059300.792855262510812No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAAAAAAAAA10442250.6865400864854118No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA9467440.6224498624717323No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA7907410.5198835447605267No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAAAAAAAAA7361710.48400574781110595No Hit
TGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAAAA6999220.4601733442630107No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAA5867610.3857740886171723No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAA4819790.31688372174976875No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAAAAAAAAAAAA4768040.3134813468329051No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAAAAAAAAAAA4401290.28936886372643617No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4395590.28899410938776715No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA4368040.2871827967508667No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAA4354690.2863050826418787No Hit
TTGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAAA4312570.28353584531824005No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA4058020.26680010550978295No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAAAAAAAA3883350.25531618615270885No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAA3842590.2526363638993492No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAA3598770.23660608269684266No Hit
TGTGCATTTATCAGATCAAAACCAACAAAAAAAAAAAAAA3397160.2233509559917433No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAA3238810.21294001747801639No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAA3200120.21039629022133124No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCAAAAAAAAAAAAAAA3137250.20626281561218685No Hit
TTGCGTGCATTTATCAGATCAAAACCAACAAAAAAAAAAA3133060.2059873383000775No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA3095890.2035435455337041No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAAA2977140.195736163478099No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAA2972470.19542912790589118No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAA2914530.19161978292650791No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA2892620.19017927984576427No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAAAAA2762050.1815947756352349No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA2611360.17168745435557903No Hit
TGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAAAA2610460.17162828261789442No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAA2541030.1670635117874046No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAA2454020.16134291968080924No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAAAAAAAA2316170.1522797818587868No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAA2310440.1519030551288616No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA2287820.1504158721217223No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAA2263960.14884716360932873No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAAA2207230.14511737174394365No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCACGAAAAAAAAAAAA2195810.14436654813910146No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAA2184840.14364531040310155No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA2170260.14268672825261125No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAA2106070.13846646842819615No Hit
TGCGTGCATTTATCAGATCAAAACCAACAAAAAAAAAAAA2093540.1376426663468763No Hit
TCTGCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA2068140.13597270841666687No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGACAAAAAAAAAA2044700.1344316133818594No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAAAAAAAAAAAA2041840.13424357874877282No Hit
TGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAAAAAA1986230.13058742282361746No Hit
CGCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA1925760.12661173951496532No Hit
TCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAAAA1852010.1217629443435895No Hit
CCTGCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA1752540.11522315240193862No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAAA1703160.111976596394311No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAAAAAAAAAAA1697120.11157948828807221No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAA1697070.11157620096931195No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAAAAAAAAA1687980.11097856641869763No Hit
TTTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAAAA1660190.10915147465174803No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAAAAAAAAA1628810.10708835339781211No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA1626190.10691609789477477No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCAAAAAAAAAAA1613680.10609361074095904No Hit
CTCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAAA1586200.104286900350323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)