FastQCFastQC Report
Thu 7 Sept 2023
SRR13890650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13890650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41421467
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC21698475.238460047781504No Hit
TTTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA20382124.920665895295306No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10904842.632654222507378Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6648081.6049841981695143No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG5432591.311539738561167No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA5036371.2158840245807807No Hit
TGCCGCCTGGATACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA4377381.0567901904584887No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC4128930.9968092148933306No Hit
TCGACGAAGCCGAGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA3990530.96339658853705No Hit
CCGCCTGGATACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2794370.6746187912658912No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2444690.5901987971599364No Hit
TTTTGGTGACTCTAGATAACCTGTAGGCACCATCAATAGATCGGAAGAGC2424790.5853945250176679No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2344690.5660567260932599No Hit
AACCCGGCGGCGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2219440.5358187820822474No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2136250.5157349931618791No Hit
TCGCCTGGATACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1957650.4726172542367946No Hit
CTTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1642560.3965480025128033No Hit
CTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1571250.37933229163515625No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCACCATCAATAGATCGGAAGAG1476730.35651320606293346No Hit
TGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1451750.35048251671047764No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG1448650.3497341125074107No Hit
TTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1440260.34770859274491656No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1428260.34481154421691534No Hit
CGTGGGGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1376570.3323325076825502Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1370730.3309226107322563No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1260640.304344604694952No Hit
TAACCCGGCGGCGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1258660.3038665916878318No Hit
TCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1225050.2957524416023218No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1212770.2927877952753339No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1151500.2779959483327812No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA1138570.2748743785438599No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1128480.2724384435732322No Hit
ATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA1078640.2604060353536006No Hit
CTCGGGCCGATCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1054210.25450812739201145No Hit
CGCGACCTCAGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1039310.2509109588030767No Hit
CCGACGAAGCCGAGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1032040.2491558302365293No Hit
AGTCCGCCCGGAGGATTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1020490.24636742102832815No Hit
TCTCGGGCCGATCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1000100.24144485273783275No Hit
CTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC971870.23462954607570996No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT880800.21264336195528757No Hit
GCCTGGATACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC873930.21098480167300687No Hit
ACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC841210.2030855160199903No Hit
GGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC839030.20255921887073675No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC823960.1989210087609886Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGGGATGCGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC822810.1986433749437218No Hit
AGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATAGATCGGAAGAGC799540.19302551500650617No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATAGA781460.18866062855765103No Hit
AGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAGATCGGAAGAG765340.18476892670170275No Hit
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG741300.1789651728172737Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG738240.17822642544263342No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA714120.172403357901351No Hit
TTTTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCAC701430.16933972908298975No Hit
ACGGGGTCGGCGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG692280.16713072958038885No Hit
TCGTGGGGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC685860.1655808086179082No Hit
TCCGCCTGGATACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC680130.16419746794578763No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA677480.1635577030625207No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT669050.16152252647159984No Hit
GGGGGGGGGATGCGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA666610.16093345993757294No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG657720.1587872298197454No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC651430.15726869354965145No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA650360.157010373389238No Hit
TTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC615850.1486789446641279No Hit
TAGCGGAGGAAAAGAAACTAACCCTGTAGGCACCATCAATAGATCGGAAG612590.14789191314735425No Hit
TTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC606240.14635889163462026No Hit
TTTTGGTGACTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC600800.14504556296859308No Hit
TTTGGTGACTCTAGATAACCTGTAGGCACCATCAATAGATCGGAAGAGCA590280.1425058170923787No Hit
TCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACA577080.13931906371157737No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT568650.13728388712065653No Hit
GCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC568010.13712937786582982No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC565700.1365716960241896No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGC533080.12869655244223968No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT506510.1222820041598237No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT505150.12195367199331689Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT495170.11954429330086258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGAGGAAAAGAAACTAACCCTGTAGGCACCATCAATAGATCGGAAGA487620.1177215669353285No Hit
CTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC481820.11632132681346123No Hit
TGGATGGTTTAGTGAGGCCCTCCTGTAGGCACCATCAATAGATCGGAAGA480440.1159881662327411No Hit
GTGGGGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG476850.11512146588144741Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG468300.11305731880524657No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT459590.11095454441533903No Hit
TTTTGGTGACTCTAGATAACCCTGTAGGCACCATCAATAGATCGGAAGAG453560.10949877753001842No Hit
TGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT442390.10680210819187065No Hit
AGATAACCTCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT436300.10533185606391006No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA434770.1049624823765899No Hit
AAGTCCTTCTGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT425950.10283315170850901No Hit
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG424330.10244205015722885No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT423550.10225374200290879No Hit
TTGGCGCTGGAGCGTCGGGCCCCTGTAGGCACCATCAATAGATCGGAAGA422890.10209440433386872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)