FastQCFastQC Report
Thu 7 Sept 2023
SRR13890654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13890654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32973538
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10196733.0923979101059764Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG9538922.892901574589903No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA9475582.873692231631316No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA9080502.7538749405659773No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG6872432.0842258419463513No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC5944351.802763779852802No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA5373251.6295642887942448No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2591840.7860363664948541Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2506480.7601489412510116No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2286580.6934591004459394No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATAGA2259150.685140308571073No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2242590.6801180995500088No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA2177770.6604599118238389No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1931070.5856423414436146No Hit
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1876090.5689683648748884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1861520.5645496701021286No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAGA1486820.4509130927958049No Hit
TTTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1447260.4389155934677073No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1180320.3579597676172936No Hit
TTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA1171680.3553394846497819No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1158590.351369634644605No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1136620.34470671603393No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC1066770.3235230626449609No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1065700.3231985600089381No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCCTGTAGGCACCATCAATAGAT1055560.32012336680401116No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1027020.3114679413534574No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA978170.2966530312883015No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA976570.2961677937017253No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG958580.2907119035876587No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCACCATCAATAGATCGGAAGAG946750.2871241781819106No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA849330.2575792746292497No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA838220.25420990613746086No Hit
TTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATA780370.23666553464781365No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT762610.23127939743681736No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCA760530.23064858857426823No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT753930.22864698352964125No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA751140.2278008504880489No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC725600.22005524551232564Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAGATC724070.21959123707016212No Hit
GATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAGAT696840.21133309989361773No Hit
GCCGCCTGGATACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC678400.20574073670832654No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG667710.202498743083014No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG621220.1883995584580581No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA617540.18728351200893273No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT612330.18570345711764386No Hit
TGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG607500.18423864615316682No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA603610.18305891227080331No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG584630.17730278140004266No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA582000.17650517211710795No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA574550.17424578460461235No Hit
TTTGGTGACTCTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC568130.1722987687884752No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC566870.17191664418904637No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA564410.1711705913996854No Hit
CGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT537000.16285786499465116No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA522330.15840884287273024No Hit
TGTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAG502540.15240706047376537No Hit
TTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT494730.15003849450429008Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACGAAGCCGAGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA493940.14979890844591806No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC492370.14932276906409012No Hit
TTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC471690.1430510732575922No Hit
TGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA446130.13529940281203673No Hit
GGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC441510.13389827928079784No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT435680.13213019482471064No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT425200.12895188863263626No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA423070.12830591609550662No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG415690.12606775772742373No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCACCATCAATAGATCGGAAGAGC406550.12329583801410696No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT402390.12203422028900872No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT401610.1217976669655528No Hit
TCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT395120.1198294220050029No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC391030.1185890334243174No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT388740.11789453712853015No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG387950.11765495107015814No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT385100.11679062161906921No Hit
TTTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC366420.11112547279579159No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAAT362640.1099790989975052No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA357360.10837781496180361No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATAGAT341990.10371650139575558No Hit
CTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG340340.10321610013459884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)