FastQCFastQC Report
Thu 14 Sept 2023
SRR13998262.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13998262.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57593965
Total Bases8.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG10874781.8881804716865735No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG9630831.6721943002187816No Hit
CGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG6279391.0902861089699243No Hit
GCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG5871381.0194436170525853No Hit
CGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT5803521.007661132550954No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT4994230.8671446739254712No Hit
CGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG4901760.8510891722769911No Hit
CCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC4807480.8347194015900798No Hit
GGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG3650450.6338250891391138No Hit
GGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC3565400.6190579169188994No Hit
CGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC3376770.5863062214938666No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG3304670.573787548747512No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC3155930.5479619262191794No Hit
GCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC2946230.5115518613799206No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC2848260.49454139856493645No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT2744020.47644228002013755No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT2450090.42540741898912493No Hit
GGGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAA2377960.4128835373636804No Hit
GGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAAA2137300.37109790930351816No Hit
GGGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG2101130.36481773741398077No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1985420.3447270907637632No Hit
CGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1921820.33368426709291504No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1839160.31933206890687243No Hit
CGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1729400.3002745166095094No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1721330.29887332813429324No Hit
CCGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1695990.29447356159625404No Hit
GCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1690540.29352728189489996No Hit
CCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1687260.2929577777810574No Hit
CCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1666020.28926989138532133No Hit
CCCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1622340.28168576343024826No Hit
CGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1569480.27250771847362826No Hit
CGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC1536910.26685261207489364No Hit
CGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1519040.2637498564302701No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1457700.2530994349842036No Hit
GCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1447310.25129542652602577No Hit
CCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACA1425310.24747558186000912No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1420750.24668383223832566No Hit
CCGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAA1397220.24259833473871786No Hit
CGCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1339290.232539989215884No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1338840.23246185602953368No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC1333510.2315364118445396No Hit
GCGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAA1304590.22651505240175773No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1299980.22571462131492423No Hit
CCGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAAA1235550.21452768532258543No Hit
CGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAAAG1200240.2083968346336287No Hit
CCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACG1190750.20674909254815152No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1184970.20574551517680714No Hit
CGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1183890.20555799552956633No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1168850.20294661081243495No Hit
GCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1168570.20289799460759475No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1150920.1998334374096314No Hit
CGGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1149760.1996320274181505No Hit
GCGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1120210.19450128151447812No Hit
CGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAAA1113680.19336748216588318No Hit
GCGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAAA1113200.19328414010044281No Hit
GGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1112190.19310877450441205No Hit
GCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1055560.18327614707547918No Hit
CCGCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAAAAAAAAAAAAAAA1029210.17870101494140922No Hit
CGGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAA1021980.17744567508071377No Hit
GGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACA1012670.1758291862697767No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG1004340.1743828541757804No Hit
GGCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC997700.17322995560385537No Hit
CGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACA992800.17237917201915165No Hit
GCCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC964520.16746893533029023No Hit
GGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACG910570.1581016344333994No Hit
GGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT894810.15536523661810747No Hit
CGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACG855070.14846520811685737No Hit
CGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC849700.1475328187597433No Hit
CGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT847880.14721681342828194No Hit
GCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACA846900.1470466567113412No Hit
CGGCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAAAAAAAAAAAAAAA830270.1441592014024386No Hit
GGGCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAAAAAAAAAAAAAAA815350.1415686522016673No Hit
CCGCGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC775630.13467209628647722No Hit
CGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGG753930.1309043404113608No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC749570.1301473166502775No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG748910.13003272131029703No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG721940.12534993900836658No Hit
GGGAGGAAAGACTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA720770.1251467927238557Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACG710220.12331500357719771No Hit
CCGGGAGCGTTTACTTTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT705740.12253714429975432No Hit
GGGGGAGCGTTTACTTTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT691350.12003861862957343No Hit
GGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT683130.1186113857589072No Hit
GGGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAA675830.11734389184700168No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG673420.11692544522676986No Hit
GCGCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAAAAAAAAAAAAAAA668110.11600347362783582No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG651130.11305524806288299No Hit
GGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC626260.10873708729725415No Hit
CCCCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAAAAAAAAAAAAAAA609710.10586352233259162No Hit
GGGCGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC590950.10260623660829742No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACA588520.10218431740200556No Hit
CCGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAA588250.10213743749019537No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG587410.10199158887567474No Hit
CGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG578700.10047927764653813No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)