FastQCFastQC Report
Thu 14 Sept 2023
SRR13998264.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13998264.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77202121
Total Bases11.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG15593832.0198706716878934No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG13402681.7360507491756605No Hit
CGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG8767631.1356721663126328No Hit
GCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG8315861.0771543439849274No Hit
CGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT8099231.0490942340819887No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT7072830.9161445188792158No Hit
CCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC6853300.8877087716281784No Hit
CGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG6640290.8601175607597621No Hit
GGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC5096810.6601904110898715No Hit
GGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG5023110.6506440412433746No Hit
CGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC4737420.613638581250896No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC4416120.5720205536840108No Hit
GCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC4353150.5638640420254775No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG3982120.5158044815893077No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT3921200.5079135066768438No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC3845430.49809900948187674No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT3382090.4380825236653796No Hit
GGGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAA3302420.4277628590022805No Hit
GGGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG2963660.3838832355396039No Hit
GGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAAA2861150.37060510293493104No Hit
CGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC2576940.3337913475201024No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2469350.31985520190565747No Hit
CCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT2350060.30440355388681617No Hit
GCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC2341540.3032999572641275No Hit
CCGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG2319260.3004140261897727No Hit
CGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACG2317130.30013812703410053No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2237600.2898365965878062No Hit
CCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG2236960.2897536973109845No Hit
CGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT2188650.28349609721214786No Hit
CCCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC2157190.27942107963588203No Hit
CGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC2129860.2758810214553561No Hit
GCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT2053260.2659590142607611No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2036210.26375052571418345No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1993890.2582688110343497No Hit
CGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1983320.2568996776655916No Hit
CCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACA1973390.2556134435736552No Hit
CCGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAA1967410.2548388534558526No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1922730.2490514476927389No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC1872460.24253996855863585No Hit
GCGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAA1790960.23198326377587478No Hit
CGCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1788180.23162317004218058No Hit
CCGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAAA1770770.22936805065239077No Hit
CCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACG1699520.22013902959997692No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1625580.21056157252467195No Hit
GCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1614100.20907456674668304No Hit
CGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1599990.207246896753005No Hit
CGGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1583480.2051083544712457No Hit
GCGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1577980.20439593881105933No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1535530.19889738521562123No Hit
CGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAAAG1532390.19849066063871482No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1527650.19787668786975426No Hit
GCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1505940.19506458896381876No Hit
CGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAAA1492270.19329391222295564No Hit
GCGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAAA1491910.19324728137974342No Hit
GGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1486810.19258667776757066No Hit
CGGGGGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAAAAAAAAAAAAAA1406850.18222944936966176No Hit
GGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACA1396980.18095098708492738No Hit
GGCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1367870.17718036529074116No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1364250.17671146625621853No Hit
CGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACA1340120.17358590445980104No Hit
GCCCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1321800.1712129126607804No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1311640.1698968866412362No Hit
GGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACG1302130.168665055199714No Hit
CCGCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAAAAAAAAAAAAAAA1282650.16614180846145407No Hit
GGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1238280.1603945570355509No Hit
GCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACA1220160.158047471260537No Hit
CGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC1210560.15680398210821178No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG1209280.1566381835545684No Hit
CGGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACG1192290.15443746681519283No Hit
CGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1159590.15020183189008499No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1110620.14385874191202597No Hit
CCGCGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1099300.14239246095324246No Hit
GCGCGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACG1054270.13655971964811692No Hit
GGGGGAGCGTTTACTTTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1007270.13047180400652464No Hit
GGGCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAAAAAAAAAAAAAAA1005620.13025807930846872No Hit
CGGCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAAAAAAAAAAAAAAA999940.1295223482266763No Hit
CCGGGAGCGTTTACTTTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT991210.12839155027878055No Hit
GGGAGGAAAGACTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA977440.12660792052591405Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT957640.12404322414924325No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG951640.12326604342903999No Hit
GGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC908500.11767811405077847No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC897790.11629084646521565No Hit
CGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGG865730.1121381108169295No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG859130.11128321202470591No Hit
GGGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAA851160.1102508569680359No Hit
GCGCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAAAAAAAAAAAAAAA836860.10839857625155143No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG835500.10822241528830535No Hit
GGGCGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACAC835380.10820687167390128No Hit
CGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG827090.10713306697882044No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG818950.10607869180174467No Hit
GGGGGAGCGTTTACTTCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT807920.104649974577771No Hit
CCGGGGGTGGGTACCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAA792800.10269147916285874No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACA787150.10195963398466734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)