FastQCFastQC Report
Thu 14 Sept 2023
SRR14029801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14029801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220280408
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGGCTGTAGGGGTTCGAGTCCCCTCCTCGGTACCATGGAATTCTCGGG12004910.5449831017200585No Hit
GCCCAGGTGGCGGAATTGGTAGACGCTGGAATTCTCGGGTGCCAAGGAAC10766720.4887733819704928RNA PCR Primer, Index 1 (100% over 24bp)
TGCTTGACGATCATAGAGCGTTGGAACCTGGAATTCTCGGGTGCCAAGGA8223800.3733332471401633RNA PCR Primer, Index 1 (100% over 22bp)
GAACTGAAGAGTTTGATCATGGCTTGGAATTCTCGGGTGCCAAGGAACTC6395890.29035219509853094RNA PCR Primer, Index 1 (100% over 26bp)
GAGTCCAGGTCGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT5831490.26473030683691123RNA PCR Primer, Index 1 (100% over 30bp)
GAACTGAAGAGTTTGATCATGGCTCTGGAATTCTCGGGTGCCAAGGAACT5672950.25753311660835493RNA PCR Primer, Index 1 (100% over 25bp)
AGGCCAGTAGCTCAATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2785360.12644610681854196RNA PCR Primer, Index 1 (100% over 30bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCACCTGGAATTCTCGGGTGCCAA2291180.10401197368401462No Hit
TGCTTGACGATCATAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2263160.102739958607667RNA PCR Primer, Index 1 (100% over 31bp)
CGAGAAACTTTGTCTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2257110.10246530867148204RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)