FastQCFastQC Report
Thu 14 Sept 2023
SRR14029802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14029802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191810301
Total Bases9.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGAAACTTTGTCTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT103489595.395413565405958RNA PCR Primer, Index 1 (100% over 30bp)
AGGTAGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACTC69940893.6463573455317184RNA PCR Primer, Index 1 (100% over 26bp)
AGAGATGGATTGGTGCCTTCGGGAACTCTGGAATTCTCGGGTGCCAAGGA14945680.7791906859058627RNA PCR Primer, Index 1 (100% over 22bp)
AAAGGTACTCCGGGGATAACAGGCTGTGGAATTCTCGGGTGCCAAGGAAC14873430.7754239434721496RNA PCR Primer, Index 1 (100% over 24bp)
AAAAGGTACTCCGGGGATAACAGGCTGTGGAATTCTCGGGTGCCAAGGAA14394040.7504310209074746RNA PCR Primer, Index 1 (100% over 23bp)
AGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT13962510.7279332719466407RNA PCR Primer, Index 1 (100% over 30bp)
AGAGAATGCGATTGCTGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG13099300.6829299537984668RNA PCR Primer, Index 1 (100% over 29bp)
AAGAGAATGCGATTGCTGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA8424830.4392271924957774RNA PCR Primer, Index 1 (100% over 28bp)
AAGGCCGAGAGCTGATGACGAGTCGTCTTGGAATTCTCGGGTGCCAAGGA7704240.40165934570948825RNA PCR Primer, Index 1 (100% over 22bp)
TAGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG7692030.40102277927190155RNA PCR Primer, Index 1 (100% over 29bp)
AGCGGAACGCTCTGGAAAGTGCGGCCTGGAATTCTCGGGTGCCAAGGAAC7342550.3828026942098381RNA PCR Primer, Index 1 (100% over 24bp)
ATGGCACCGTAACTTCGGGAGAAGGTGGAATTCTCGGGTGCCAAGGAACT6926970.36113649600080655RNA PCR Primer, Index 1 (100% over 25bp)
TCCGGAGATCTCTGAATGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT5926190.3089609874497825RNA PCR Primer, Index 1 (100% over 30bp)
ACTGGAACTGAGACACGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG5924730.3088848705784576RNA PCR Primer, Index 1 (100% over 29bp)
TTTAAGAGAATGCGATTGCTGAGCCTGGAATTCTCGGGTGCCAAGGAACT5831800.3040399795837868RNA PCR Primer, Index 1 (100% over 25bp)
TTAAGAGAATGCGATTGCTGAGCCTGGAATTCTCGGGTGCCAAGGAACTC5350530.2789490435135702RNA PCR Primer, Index 1 (100% over 26bp)
AGGAACTAGGCAAAATGGCACCGTGGAATTCTCGGGTGCCAAGGAACTCC5263120.27439193685431945RNA PCR Primer, Index 1 (100% over 27bp)
ACACGGTCCAGACTCCTACGGGATGGAATTCTCGGGTGCCAAGGAACTCC5056430.26361618607751414RNA PCR Primer, Index 1 (100% over 27bp)
CGAGAAACTTTGTCTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC4762080.24827029493061484RNA PCR Primer, Index 1 (100% over 31bp)
AGGTAGCGAAATTCCTTGTCGGGTGGAATTCTCGGGTGCCAAGGAACTCC4564050.23794603189742142RNA PCR Primer, Index 1 (100% over 27bp)
AATGGGTGTGAAAAACACCCACGCCGAAAGACTGGAATTCTCGGGTGCCA4355530.2270748743572432No Hit
AGACACGGTCCAGACTCCTACGGGATGGAATTCTCGGGTGCCAAGGAACT4241200.22111429771438604RNA PCR Primer, Index 1 (100% over 25bp)
CGGAGATCTCTGAATGGGGGAACCTGGAATTCTCGGGTGCCAAGGAACTC4229250.22049128633607637RNA PCR Primer, Index 1 (100% over 26bp)
ATACCAGGCCGCTGCGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT4215230.21976035583198425RNA PCR Primer, Index 1 (100% over 30bp)
AGAGAACTCGGGTGAAGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAG4143360.21601342463875287RNA PCR Primer, Index 1 (100% over 29bp)
ACGGTCCAGACTCCTACGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG4060620.21169978769805486RNA PCR Primer, Index 1 (100% over 29bp)
ACGAGAGGACCGGAGTGGACGTGGAATTCTCGGGTGCCAAGGAACTCCAG3949560.20590969199302805RNA PCR Primer, Index 1 (100% over 29bp)
ACGGATAAAAGGTACTCCGGGGATTGGAATTCTCGGGTGCCAAGGAACTC3876280.20208925067064046RNA PCR Primer, Index 1 (100% over 26bp)
ACGAGAAACTTTGTCTGAACATGGGTGGAATTCTCGGGTGCCAAGGAACT3806180.19843459815017964RNA PCR Primer, Index 1 (100% over 25bp)
ACGGATAAAAGGTACTCCGGGGATAACTGGAATTCTCGGGTGCCAAGGAA3743090.1951454108817649RNA PCR Primer, Index 1 (100% over 23bp)
TAGAACCTGAAACCGTATGCGTACTGGAATTCTCGGGTGCCAAGGAACTC3675480.19162057412130332RNA PCR Primer, Index 1 (100% over 26bp)
GAGAAACTTTGTCTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3554740.185325813132424RNA PCR Primer, Index 1 (100% over 32bp)
AGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACTCCA3511510.18307202385340085RNA PCR Primer, Index 1 (100% over 28bp)
ACGATGGCGGCGCTGTCTCCACCCGAGTGGAATTCTCGGGTGCCAAGGAA3451910.17996478718835857RNA PCR Primer, Index 1 (100% over 23bp)
GTTAGGTAACACTGACTGGAGGACCTGGAATTCTCGGGTGCCAAGGAACT3399400.1772271865628322RNA PCR Primer, Index 1 (100% over 25bp)
ATCCGGAGATCTCTGAATGGGGGAACCTGGAATTCTCGGGTGCCAAGGAA3384620.1764566335777764RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC3273470.170661845736846RNA PCR Primer, Index 6 (100% over 50bp)
ACACGGTCCAGACTCCTACGGGAGGTGGAATTCTCGGGTGCCAAGGAACT3262670.17009878942841553RNA PCR Primer, Index 1 (100% over 25bp)
TAACGGTCCTAAGGTAGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGT3182080.1658972424009699RNA PCR Primer, Index 1 (100% over 30bp)
TGTGTAAGCGTTGTGAGGCGTTGAGCTGGAATTCTCGGGTGCCAAGGAAC3123820.1628598664260477RNA PCR Primer, Index 1 (100% over 24bp)
AGGTTAGGTAACACTGACTGGAGGACCTGGAATTCTCGGGTGCCAAGGAA3120950.16271023942556662RNA PCR Primer, Index 1 (100% over 23bp)
TCCGGAGATCTCTGAATGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG3081290.1606425715373858RNA PCR Primer, Index 1 (100% over 29bp)
ATAGAACCTGAAACCGTATGCGTACTGGAATTCTCGGGTGCCAAGGAACT3057630.15940906114317602RNA PCR Primer, Index 1 (100% over 25bp)
TAAGAGAATGCGATTGCTGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC3007270.15678355043090203RNA PCR Primer, Index 1 (100% over 27bp)
ACGGATAAAAGGTACTCCGGGGATGGAATTCTCGGGTGCCAAGGAACTCC2915190.15198297405309844RNA PCR Primer, Index 1 (100% over 27bp)
AGATACCAGGCCGCTGCGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA2860170.14911451497070535RNA PCR Primer, Index 1 (100% over 28bp)
TGGCGGCGCTGTCTCCACCCGAGACTCTGGAATTCTCGGGTGCCAAGGAA2831960.1476437910391476RNA PCR Primer, Index 1 (100% over 23bp)
AAGGTAGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACT2819870.14701348078276566RNA PCR Primer, Index 1 (100% over 25bp)
ACACTGGAACTGAGACACGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC2797050.1458237636569894RNA PCR Primer, Index 1 (100% over 27bp)
CGGTCCAGACTCCTACGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT2791110.14551408268735264RNA PCR Primer, Index 1 (100% over 30bp)
CGGCGCTGTCTCCACCCGAGACTCAGTGTGGAATTCTCGGGTGCCAAGGA2763060.14405170033073458RNA PCR Primer, Index 1 (100% over 22bp)
AAGCGTTGTGAGGCGTTGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2642120.13774651237318064RNA PCR Primer, Index 1 (100% over 28bp)
ATCCGGAGATCTCTGAATGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG2570870.13403190478284063RNA PCR Primer, Index 1 (100% over 29bp)
ATACGGTGGATGCCTTGGCAGTCTGGAATTCTCGGGTGCCAAGGAACTCC2569080.1339385834132026RNA PCR Primer, Index 1 (100% over 27bp)
TCCGGAGATCTCTGAATGGGGGAACCTGGAATTCTCGGGTGCCAAGGAAC2539210.13238131564164535RNA PCR Primer, Index 1 (100% over 24bp)
CGAGAAACTTTGTCTGAACATGGGTGGAATTCTCGGGTGCCAAGGAACTC2500850.13038142304984965RNA PCR Primer, Index 1 (100% over 26bp)
TGGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG2498220.13024430841177814Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGAGATCTCTGAATGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2489370.12978291504792538RNA PCR Primer, Index 1 (100% over 31bp)
ACGGTCCAGACTCCTACGGGAGGTGGAATTCTCGGGTGCCAAGGAACTCC2452880.12788051461323757RNA PCR Primer, Index 1 (100% over 27bp)
AACGGAGGAGTACGAAGGTGCGCTCTGGAATTCTCGGGTGCCAAGGAACT2370360.1235783473380817RNA PCR Primer, Index 1 (100% over 25bp)
TGGCGGCGCTGTCTCCACCCGAGATGGAATTCTCGGGTGCCAAGGAACTC2361280.1231049629602531RNA PCR Primer, Index 1 (100% over 26bp)
ACGAGAGGACCGGAGTGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT2341670.1220825986817048RNA PCR Primer, Index 1 (100% over 30bp)
AATGGCACCGTAACTTCGGGAGAAGGTGGAATTCTCGGGTGCCAAGGAAC2337040.12184121435688691RNA PCR Primer, Index 1 (100% over 24bp)
AGTACGAGAGGACCGGAGTGGACGTGGAATTCTCGGGTGCCAAGGAACTC2326050.12126825242821553RNA PCR Primer, Index 1 (100% over 26bp)
TTGTCTGAACATGGGGGGACCATGGAATTCTCGGGTGCCAAGGAACTCCA2325710.12125052658146865RNA PCR Primer, Index 1 (100% over 28bp)
ACGGTCCAGACTCCTACGGGAGGCAGCTGGAATTCTCGGGTGCCAAGGAA2317020.12079747479255558RNA PCR Primer, Index 1 (100% over 23bp)
ATAACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2298390.11982620266051301RNA PCR Primer, Index 1 (100% over 30bp)
ATCCGGAGATCTCTGAATGGGGTGGAATTCTCGGGTGCCAAGGAACTCCA2256280.11763080440606785RNA PCR Primer, Index 1 (100% over 28bp)
AGGTAACACTGACTGGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCA2233470.11644160862872531RNA PCR Primer, Index 1 (100% over 28bp)
ATAACGGTCCTAAGGTAGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG2164510.11284638982970993RNA PCR Primer, Index 1 (100% over 29bp)
AGAACCTGAAACCGTATGCGTACTGGAATTCTCGGGTGCCAAGGAACTCC2129810.11103731076466013RNA PCR Primer, Index 1 (100% over 27bp)
AAGGTACTCCGGGGATAACAGGCTGTGGAATTCTCGGGTGCCAAGGAACT2117890.11041586343165166RNA PCR Primer, Index 1 (100% over 25bp)
ACTTTGTCTGAACATGGGGGGACCTGGAATTCTCGGGTGCCAAGGAACTC2115800.11030690160900171RNA PCR Primer, Index 1 (100% over 26bp)
ATCCGGAGATCTCTGAATGGGGGTGGAATTCTCGGGTGCCAAGGAACTCC2113110.11016665888032781RNA PCR Primer, Index 1 (100% over 27bp)
TGCTGAGAACTTTCCAGAGATGGTGGAATTCTCGGGTGCCAAGGAACTCC2076020.10823297753961608RNA PCR Primer, Index 1 (100% over 27bp)
AAGGCCGAGAGCTGATGACGAGTCGTGGAATTCTCGGGTGCCAAGGAACT2067660.10779713024901619RNA PCR Primer, Index 1 (100% over 25bp)
AGAATGCGATTGCTGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2053530.10706046491215297RNA PCR Primer, Index 1 (100% over 31bp)
ATGTATCACTCTGGGGGGTAGAGTGGAATTCTCGGGTGCCAAGGAACTCC1996410.1040825226586762RNA PCR Primer, Index 1 (100% over 27bp)
AAGGCGCTTGAGAGAACTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1971070.10276142572759948RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph