FastQCFastQC Report
Thu 7 Sept 2023
SRR14029803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14029803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61393135
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACTGAAGAGTTTGATCATGGCTTGGAATTCTCGGGTGCCAAGGAACTC1761690.2869522789478009RNA PCR Primer, Index 1 (100% over 26bp)
ATAGGCTGTAGGGGTTCGAGTCCCCTCCTCGGTACCATGGAATTCTCGGG1751770.28533646310780514No Hit
GAACTGAAGAGTTTGATCATGGCTCTGGAATTCTCGGGTGCCAAGGAACT1676110.2730126096346114RNA PCR Primer, Index 1 (100% over 25bp)
GCCCAGGTGGCGGAATTGGTAGACGCTGGAATTCTCGGGTGCCAAGGAAC954420.15546037842830474RNA PCR Primer, Index 1 (100% over 24bp)
TGCTTGACGATCATAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC952770.15519161873717638RNA PCR Primer, Index 1 (100% over 31bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTTGGAATTCTCGGG848090.13814085239334983No Hit
GAGTCCAGGTCGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT759070.12364085984532311RNA PCR Primer, Index 1 (100% over 30bp)
TGCTTGACGATCATAGAGCGTTGGAACCTGGAATTCTCGGGTGCCAAGGA701390.11424567258212176RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)