FastQCFastQC Report
Thu 14 Sept 2023
SRR14029804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14029804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133606235
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACTC49080963.67355310925422RNA PCR Primer, Index 1 (100% over 26bp)
ACGAGAAACTTTGTCTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT29099772.1780248504121085RNA PCR Primer, Index 1 (100% over 30bp)
AATGGGTGTGAAAAACACCCACGCCGAAAGACTGGAATTCTCGGGTGCCA22225191.6634844923217842No Hit
AGCGGAACGCTCTGGAAAGTGCGGCCTGGAATTCTCGGGTGCCAAGGAAC18530581.3869547330631689RNA PCR Primer, Index 1 (100% over 24bp)
AGAGATGGATTGGTGCCTTCGGGAACTCTGGAATTCTCGGGTGCCAAGGA14427651.079863525830213RNA PCR Primer, Index 1 (100% over 22bp)
ACTGACTGGAGGACCGAACCCTGGAATTCTCGGGTGCCAAGGAACTCCAG9399360.7035120778607376RNA PCR Primer, Index 1 (100% over 29bp)
ACTGGAACTGAGACACGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG8710370.6519433767443562RNA PCR Primer, Index 1 (100% over 29bp)
AACGGATAAAAGGTACTCCGGGGATAACTGGAATTCTCGGGTGCCAAGGA7473590.5593743435701186RNA PCR Primer, Index 1 (100% over 22bp)
GAGAAACTTTGTCTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA6799280.5089043935711534RNA PCR Primer, Index 1 (100% over 32bp)
AAGCGTTGTGAGGCGTTGAGCTAACCTGGAATTCTCGGGTGCCAAGGAAC6520010.48800192595802133RNA PCR Primer, Index 1 (100% over 24bp)
AGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT6372650.4769725005722974RNA PCR Primer, Index 1 (100% over 30bp)
AAACTCCGAATACCCAGAAGTGCCGAGCTGGAATTCTCGGGTGCCAAGGA6004420.44941166106506936RNA PCR Primer, Index 1 (100% over 22bp)
AAGGTAGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACT5822610.43580376320012315RNA PCR Primer, Index 1 (100% over 25bp)
ATACCAGGCCGCTGCGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT5809810.4348457240786704RNA PCR Primer, Index 1 (100% over 30bp)
GATGGCGGCGCTGTCTCCACCCGAGACTCTGGAATTCTCGGGTGCCAAGG5449050.40784399021497764Illumina Small RNA Adapter 2 (100% over 21bp)
AGATACCAGGCCGCTGCGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA5242450.3923806400202805RNA PCR Primer, Index 1 (100% over 28bp)
GAAATAGAACCTGAAACCGTATGCGTACTGGAATTCTCGGGTGCCAAGGA5164020.3865104049971919RNA PCR Primer, Index 1 (100% over 22bp)
ACGGTCCAGACTCCTACGGGAGGCAGCTGGAATTCTCGGGTGCCAAGGAA4966240.3717072036346208RNA PCR Primer, Index 1 (100% over 23bp)
ATGTATCACTCTGGGGGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACTC4497080.3365920759611256RNA PCR Primer, Index 1 (100% over 26bp)
ACACTGGAACTGAGACACGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC4070700.30467889466386056RNA PCR Primer, Index 1 (100% over 27bp)
AGAACCTGAAACCGTATGCGTACTGGAATTCTCGGGTGCCAAGGAACTCC3922100.29355665923824587RNA PCR Primer, Index 1 (100% over 27bp)
ATGGCTCAGATTGAACGCTGGCGGCTGGAATTCTCGGGTGCCAAGGAACT3760500.2814614153299058RNA PCR Primer, Index 1 (100% over 25bp)
AGGTTGAAGGTTAGGTAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3707720.2775110008900408RNA PCR Primer, Index 1 (100% over 31bp)
ACTGACTGACCGATAGTGAACCTGGAATTCTCGGGTGCCAAGGAACTCCA3636120.2721519695544149RNA PCR Primer, Index 1 (100% over 28bp)
ATGAGCAGGTTGAAGGTTAGGTAACTGGAATTCTCGGGTGCCAAGGAACT3566010.26690445996027057RNA PCR Primer, Index 1 (100% over 25bp)
ACTACTGACTGACCGATAGTGAACCTGGAATTCTCGGGTGCCAAGGAACT3501470.26207384707757087RNA PCR Primer, Index 1 (100% over 25bp)
AGGTGGGAGGCTTTGAAGCGTGGACGCCTGGAATTCTCGGGTGCCAAGGA3480970.26053948754711936RNA PCR Primer, Index 1 (100% over 22bp)
ATCTCTTTTTAAGAGAATGCGATTGCTGAGCCTGGAATTCTCGGGTGCCA3313490.24800414441736193No Hit
AAGCGTTGTGAGGCGTTGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2867850.2146494136295361RNA PCR Primer, Index 1 (100% over 28bp)
ACGGATAAAAGGTACTCCGGGGATAACTGGAATTCTCGGGTGCCAAGGAA2847550.21313002346035723RNA PCR Primer, Index 1 (100% over 23bp)
AATGGGTGTGAAAAACACCCACGCCGAAAGACCTGGAATTCTCGGGTGCC2827690.2116435658859783No Hit
GAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACTCC2827540.21163233886502378RNA PCR Primer, Index 1 (100% over 27bp)
AACCTAGGTATCTTGTACTGAATCCTGGAATTCTCGGGTGCCAAGGAACT2815760.21075064348606187RNA PCR Primer, Index 1 (100% over 25bp)
AAACAGACTTTGATCCGGAGATCTCTGGAATTCTCGGGTGCCAAGGAACT2729730.20431157273461076RNA PCR Primer, Index 1 (100% over 25bp)
ACGTGCTACAATGGTCGGTACTGGAATTCTCGGGTGCCAAGGAACTCCAG2612730.19555449639008238RNA PCR Primer, Index 1 (100% over 29bp)
AAAAGGTACTCCGGGGATAACAGGCTGTGGAATTCTCGGGTGCCAAGGAA2589320.19380233265311309RNA PCR Primer, Index 1 (100% over 23bp)
AGGTAGCGAAATTCCTTGTCGGGTATGGAATTCTCGGGTGCCAAGGAACT2576390.19283456344683314RNA PCR Primer, Index 1 (100% over 25bp)
AAACCGATGCAAACTCCGAATACCCTGGAATTCTCGGGTGCCAAGGAACT2442500.18281332454282542RNA PCR Primer, Index 1 (100% over 25bp)
ACTGGGAACTTGATTCCCCTGAAGGGCTGGAATTCTCGGGTGCCAAGGAA2436420.1823582559601354RNA PCR Primer, Index 1 (100% over 23bp)
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCTGGAATTCTCGGGT2313300.17314311716066247No Hit
AGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACTCCA2267890.16974432368369635RNA PCR Primer, Index 1 (100% over 28bp)
AGATGAGCCTAGGTCGGATTAGCTTGGAATTCTCGGGTGCCAAGGAACTC2264630.16950032309495136RNA PCR Primer, Index 1 (100% over 26bp)
AGACTTTGATCCGGAGATCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG2222320.16633355471771208RNA PCR Primer, Index 1 (100% over 29bp)
ATAGAACCTGAAACCGTATGCGTACTGGAATTCTCGGGTGCCAAGGAACT2204360.16498930607542378RNA PCR Primer, Index 1 (100% over 25bp)
AGATTGAACGCTGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2194330.164238592607598RNA PCR Primer, Index 1 (100% over 32bp)
TTTAAGAGAATGCGATTGCTGAGCCTGGAATTCTCGGGTGCCAAGGAACT2158500.1615568315355941RNA PCR Primer, Index 1 (100% over 25bp)
AGAGAACTCGGGTGAAGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAG2102890.15739460063372043RNA PCR Primer, Index 1 (100% over 29bp)
CGGCGCTGTCTCCACCCGAGACTCAGTGTGGAATTCTCGGGTGCCAAGGA2042010.1528379270623111RNA PCR Primer, Index 1 (100% over 22bp)
AGTCAGAGGCGATGAAAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT2012110.15060000755204275RNA PCR Primer, Index 1 (100% over 30bp)
AGGTAGCGAAATTCCTTGTCGGGTAATGGAATTCTCGGGTGCCAAGGAAC1963180.1469377533166772RNA PCR Primer, Index 1 (100% over 24bp)
ACTCTAAGGAGACTGCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCC1962170.14686215804225006RNA PCR Primer, Index 1 (100% over 27bp)
AAAGGTACTCCGGGGATAACAGGCTGTGGAATTCTCGGGTGCCAAGGAAC1952940.14617132201951505RNA PCR Primer, Index 1 (100% over 24bp)
AAAAGCGCGCTTGACTGCGAGACAGACTGGAATTCTCGGGTGCCAAGGAA1935300.14485102435526306RNA PCR Primer, Index 1 (100% over 23bp)
CGGAGATCTCTGAATGGGGGAACCTGGAATTCTCGGGTGCCAAGGAACTC1890600.14150537211081504RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTCGGAGATAGCTGGTTCTCTGGAATTCTCGGGTGCCAAGGAACTCC1878930.1406319098805531RNA PCR Primer, Index 1 (100% over 27bp)
TAGAACCTGAAACCGTATGCGTACTGGAATTCTCGGGTGCCAAGGAACTC1841680.1378438663435131RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGGAGGAAGGTGGGGATGACGTCTGGAATTCTCGGGTGCCAAGGAA1839120.13765225851922255RNA PCR Primer, Index 1 (100% over 23bp)
ACGAATGGCGTAACGATGGCGGCGCTGGAATTCTCGGGTGCCAAGGAACT1835990.13741798801530483RNA PCR Primer, Index 1 (100% over 25bp)
AAGGCGCTTGAGAGAACTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1833830.1372563189135597RNA PCR Primer, Index 1 (100% over 31bp)
TGTGTAAGCGTTGTGAGGCGTTGAGCTGGAATTCTCGGGTGCCAAGGAAC1806520.13521225263177275RNA PCR Primer, Index 1 (100% over 24bp)
GAGAGGACCGGAGTGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1803940.13501914787135497RNA PCR Primer, Index 1 (100% over 32bp)
AAGGAGACTGCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1791380.13407907198342953RNA PCR Primer, Index 1 (100% over 32bp)
TGGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG1748190.13084643841659035Illumina Small RNA Adapter 2 (100% over 21bp)
ATAGAACCTGAAACCGTATGCGTACATGGAATTCTCGGGTGCCAAGGAAC1736730.12998869401566476RNA PCR Primer, Index 1 (100% over 24bp)
ACGAGAGGACCGGAGTGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1733520.12974843576723796RNA PCR Primer, Index 1 (100% over 30bp)
AAATAGAACCTGAAACCGTATGCGTACTGGAATTCTCGGGTGCCAAGGAA1698340.12711532511937035RNA PCR Primer, Index 1 (100% over 23bp)
AGTTCGGTCCCTATCTGCCGTGGACTGGAATTCTCGGGTGCCAAGGAACT1681550.12585864724052737RNA PCR Primer, Index 1 (100% over 25bp)
ACGGATAAAAGGTACTCCGGGGATGGAATTCTCGGGTGCCAAGGAACTCC1659040.12417384562928518RNA PCR Primer, Index 1 (100% over 27bp)
ATGTTCGGAAAAGATAACCGCTGAAAGCTGGAATTCTCGGGTGCCAAGGA1645530.12316266527531443RNA PCR Primer, Index 1 (100% over 22bp)
AACGGAGGAGTACGAAGGTGCGCTCTGGAATTCTCGGGTGCCAAGGAACT1627340.12180120186756253RNA PCR Primer, Index 1 (100% over 25bp)
TTAAGAGAATGCGATTGCTGAGCCTGGAATTCTCGGGTGCCAAGGAACTC1621550.12136783885871794RNA PCR Primer, Index 1 (100% over 26bp)
ACGGATAAAAGGTACTCCGGGGATTGGAATTCTCGGGTGCCAAGGAACTC1603240.11999739383420244RNA PCR Primer, Index 1 (100% over 26bp)
AGGTTAGGTAACACTGACTGGAGGACCTGGAATTCTCGGGTGCCAAGGAA1600780.11981327069054824RNA PCR Primer, Index 1 (100% over 23bp)
AAATACTACTGACTGACCGATAGTGAACCTGGAATTCTCGGGTGCCAAGG1567650.11733359599572579Illumina Small RNA Adapter 2 (100% over 21bp)
AATGGGCGAAAGCCTGATCCAGCCTGGAATTCTCGGGTGCCAAGGAACTC1542980.1154871252827385RNA PCR Primer, Index 1 (100% over 26bp)
AGGTAACACTGACTGGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCA1532310.11468850985884005RNA PCR Primer, Index 1 (100% over 28bp)
AAACGATGTGGGAAGGCTTAGACTGGAATTCTCGGGTGCCAAGGAACTCC1521250.11386070418045985RNA PCR Primer, Index 1 (100% over 27bp)
AGAGAATGCGATTGCTGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1449260.10847248259035216RNA PCR Primer, Index 1 (100% over 29bp)
AGATTGAACGCTGGCGGCAGGCCTAACTGGAATTCTCGGGTGCCAAGGAA1429360.10698303114371871RNA PCR Primer, Index 1 (100% over 23bp)
AGATATAGGAAGGAACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1421820.106418686223738RNA PCR Primer, Index 1 (100% over 31bp)
AACTTTGTCTGAACATGGGGGGACCTGGAATTCTCGGGTGCCAAGGAACT1419050.10621136057011112RNA PCR Primer, Index 1 (100% over 25bp)
AGCAGTGGGGAATATTGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1405400.10518970166324947RNA PCR Primer, Index 1 (100% over 30bp)
AAGTACCCTGAGGAAAAGAAATCTGGAATTCTCGGGTGCCAAGGAACTCC1372570.10273248101033608RNA PCR Primer, Index 1 (100% over 27bp)
TGGCGGCGCTGTCTCCACCCGAGACTCTGGAATTCTCGGGTGCCAAGGAA1350690.10109483288710291RNA PCR Primer, Index 1 (100% over 23bp)
TAGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1337700.10012257287244117RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph