FastQCFastQC Report
Thu 14 Sept 2023
SRR14066130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14066130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22466073
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCGAATCTCGTAT404010.179831161413924TruSeq Adapter, Index 3 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCGAATCTCGGTT390930.1740090491115203TruSeq Adapter, Index 3 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCGAATCTCGTTT385300.17150304817401776TruSeq Adapter, Index 3 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCGAATCTCGGAT256230.1140519751716288TruSeq Adapter, Index 3 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)