FastQCFastQC Report
Sat 23 Sept 2023
SRR14066133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14066133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113339502
Total Bases17 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA48282424.259981661115822RNA PCR Primer, Index 1 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA42638043.7619752379007276No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT31182862.7512790730278662No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC28368442.50296141234148No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC24603342.1707647877259952No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG22569381.9913074966572555No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC17832421.5733631862966895No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG12188641.0754097013766657No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG9931530.8762637760663532Illumina Small RNA Adapter 2 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC9434720.8324299854432041No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG9402180.8295589652405566No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG7725330.6816096650927582No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT7682840.6778607514968612No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA7660280.6758702716022169RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG6498740.5733870261755694No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG6464080.5703289573303402Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGAACT6348390.5601215717358632RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT5284570.4662602099663364RNA PCR Primer, Index 1 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG4903890.43267262635404913No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA4709570.41552767719060557RNA PCR Primer, Index 1 (100% over 23bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG4519700.39877535371560036No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAAGTGGAATTCTCGGGTGCCAAGG4401920.3883835663932951Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA4221970.37250648939678593No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCAA4196730.37027955178416083No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCAA4146400.3658389111326782No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCC3964640.34980213694604023No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGGAATTCTCGGGTGCCAAGG3564270.31447729495052834Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCTGGAATTCTCGGGTGCC2905360.2563413416092123No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2749150.24255885648765246No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCCA2706940.23883464742945493No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA2697620.2380123392460292No Hit
CGCGACCGGCTCCGGGACGGCTGGTGGAATTCTCGGGTGCCAAGGAACTC2669850.2355621784891908RNA PCR Primer, Index 1 (100% over 26bp)
AGACCCTGTTGAGCTTGACTCTAGTCTGGCACGTGGAATTCTCGGGTGCC2549240.22492069887513713No Hit
TGGAGCACGAGCGCACGTGTTAGGACCCGATGGAATTCTCGGGTGCCAAG2508780.22135089317756135No Hit
ATGGATTTTTGGAGCAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2410010.21263636750406759RNA PCR Primer, Index 1 (100% over 32bp)
CCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAAC2356340.20790103701002674RNA PCR Primer, Index 1 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2192840.19347535160336243No Hit
CGGCTCCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCC2171210.19156692606607711RNA PCR Primer, Index 20 (100% over 39bp)
CGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCTGGAATTCTCGG2166640.19116371271862478No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATGGAATTCTCGGGTGC2045500.18047547094392563No Hit
ATGGATTTTTGGAGCAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2030040.17911142754094683RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1934070.17064394724444792No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC1792710.1581716849258787No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA1699230.14992389855392166No Hit
AGCACGAGCGCACGTGTTAGGACCCGATGGAATTCTCGGGTGCCAAGGAA1678660.14810899733792723RNA PCR Primer, Index 1 (100% over 23bp)
AGCGGAGGAGAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCCA1657160.14621204176457384No Hit
CACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGT1641890.144864762155034No Hit
CCCTTGTAAATTTAACTGTTAGTCCAATGGAATTCTCGGGTGCCAAGGAA1601040.1412605465656625RNA PCR Primer, Index 1 (100% over 23bp)
CGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCCA1585240.13986650479547721No Hit
GACCCTGTTGAGCTTGACTCTAGTCTGGCACGTGGAATTCTCGGGTGCCA1518990.1340212347147952No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1487760.13126579645638464RNA PCR Primer, Index 1 (100% over 24bp)
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCC1476350.13025908654513058No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCTGGAATTCTCGGGTGCCAAGGA1465230.12927796347649384RNA PCR Primer, Index 1 (100% over 22bp)
AGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGTGGAATTCTCGGGTGC1426840.12589079489691068No Hit
ATTCCCCATGAACGTACACTGACTGGCTCAGCGTGGAATTCTCGGGTGCC1422550.12551228608715784No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAAGAGTGGAATTCTCGGGTGCCAA1404200.12389325656292367No Hit
CACTCGCCGAATCCCGGGGCCGAGGGATGGAATTCTCGGGTGCCAAGGAA1372110.12106194008157896RNA PCR Primer, Index 1 (100% over 23bp)
CGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGAACTCC1348870.11901146345252162RNA PCR Primer, Index 1 (100% over 27bp)
AGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTGGAATTCTCGGGTGC1308860.11548136147624859No Hit
CGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGTGGAATTCTCGGG1281720.1130867859292341No Hit
ATTCCCCATGAACTACACTGACTGGCTCAGCGTGGAATTCTCGGGTGCCA1253550.11060133297568223No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGATGGAATTCTCGGGTGCCAAGG1238550.10927787559892402Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGG1202860.10612892934715737No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT1147950.10128419304330452No Hit
GCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGGA1143950.10093127107616902RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)