FastQCFastQC Report
Thu 7 Sept 2023
SRR14189729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14189729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56429503
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAAACTGTAGGCACCATCA52616689.32432100279175No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTAACTGTAGGCACCATCAA31803865.636034044106325No Hit
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAACTGTAGGCACCATC28485505.047979954741051No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAACTGTAGGCACCATC17068753.024791836284647No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAACTGTAGGCACCATCA14092162.4973035824894647No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAACTGTAGGCACCATCA13361532.3678269858233554No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAAACTGTAGGCACCATCAA12571762.2278700558464957No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAACTGTAGGCACCATCAA8570261.5187551802467585No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTAACTGTAGGCACCATCAAT5807121.0290928842665865No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCAGAACTGTAGGCACCATCAA4857890.8608776866243179No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCAAACTGTAGGCACCATCAAT4739760.8399436018424618No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAACTGTAGGCACCAT4495480.796654189919057No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAACTGTAGGCACCATC3304400.5855802061556347No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAAACTGTAGGCACCATC3145090.5573485203298707No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTAACTGTAGGCACCATCAAT3017210.5346866159710816No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAACTGTAGGCACCATCA1832530.32474679069918444No Hit
GTTGACTCTGAATAAAGCAGTTTACTGTCAGAACTGTAGGCACCATCAAT1715320.30397574120048515No Hit
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAACTGTAGGCACCATCAA1242450.22017737778055568No Hit
TTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGAACTGTAGGCACCA1128280.19994505356533088No Hit
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTAACTGTAGGCACCATCA997680.17680113184764362No Hit
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAACTGTAGGCACCAT961930.17046579339888923No Hit
GTTGACTCTGAATAAAGCAGTTTACTGTCAAACTGTAGGCACCATCAATA943660.16722812533011322No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGAACTGTAGGCACCATCAAT809720.1434923146496612No Hit
TTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGGAACTGTAGGCACC782830.1387270768626121No Hit
GTTGACTCTGAATAAAGCAGTTTACTGTCAAACTGTAGGCACCATCAATT766740.1358757315300119No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTAACTGTAGGCACCATCAAT727680.1289538204864218No Hit
GTTGACTCTGAATAAAGCAGTTTACTGTCAAACTGTAGGCACCATCAATC692340.12269113906603075No Hit
ATTGTTGACTCTGAATAAAGCAGTTTACTGTCAAACTGTAGGCACCATCA675560.11971751727106299No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAACTGTAGGCACCATCAA574980.10189350772768634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)