FastQCFastQC Report
Sat 23 Sept 2023
SRR14243834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14243834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102813634
Total Bases15.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAGG90778428.829414589119573Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAAC27147962.640501939655202RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA19809471.9267357090013957No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAAGTGGAATTCTCGGGTGCCAAGG15000291.4589786798120568Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAGGA11104711.0800814607914744RNA PCR Primer, Index 1 (100% over 22bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG10400801.0116168056077077No Hit
CCCTTGTAAATTTAACTGTTAGTCCAATGGAATTCTCGGGTGCCAAGGAA9087070.883839005243215RNA PCR Primer, Index 1 (100% over 23bp)
AGCATAATTTTTTGGTATTTGGGCAGCTGGTGGAATTCTCGGGTGCCAAG7325400.7124930532073207No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAAGATGGAATTCTCGGGTGCCAAG6055350.5889637166214745No Hit
CATAATTTTTTGGTATTTGGGCAGCTGGTGGAATTCTCGGGTGCCAAGGA5691680.5535919487098375RNA PCR Primer, Index 1 (100% over 22bp)
ATGGATTTTTGGAGCAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5682680.5527165784257757RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC5348030.5201673933634132No Hit
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA4779400.46486052618274337RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA4400820.4280385615005107No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC4261970.41453354328473596No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAAGAGTGGAATTCTCGGGTGCCAA4249730.41334303969841196No Hit
CGCCGCGACCGGCTCCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT3907270.3800342277562137RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATGC3837400.37323843645094773RNA PCR Primer, Index 35 (100% over 50bp)
AAGCATAATTTTTTGGTATTTGGGCAGCTGGTGGAATTCTCGGGTGCCAA3728180.36261533173703403No Hit
TACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTGGAATTCTCGGG3699520.35982776369912184No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG3226690.31383872687546477No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG3218100.3130032345710103No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA3093140.30084920449363745No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAATGGAATTCTCGGGTGCCAAGGA3081800.2997462379357197RNA PCR Primer, Index 1 (100% over 22bp)
GGGCTTCTGTCGTGAGTGGCACACGTAGTGGAATTCTCGGGTGCCAAGGA3017530.2934951214738699RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA2775440.26994863346625797RNA PCR Primer, Index 1 (100% over 22bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAAGTGGAATTCTCGGGTGCCAAG2609190.25377859905233974No Hit
GGGAGAGCGGCCGCCAGATCTTCCGGATGGCTCTGGAATTCTCGGGTGCC2506590.24379937781403585No Hit
ATGGATTTTTGGAGCAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2350190.22858738754434066RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTGGAATTCTCGGGTGCCA2297720.2234839787882607No Hit
GGGAGAGCGGCCGCCAGATCTTCCGGATGGCTCGTGGAATTCTCGGGTGC2281710.22192679231627976No Hit
CCCTTGTAAATTTAACTGTTAGTCCTGGAATTCTCGGGTGCCAAGGAACT2223520.2162670371129961RNA PCR Primer, Index 1 (100% over 25bp)
CATAATTTGTGGTAGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2068350.20117468078212275RNA PCR Primer, Index 1 (100% over 31bp)
TTTATAGGTAGAGGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2004000.19491578325108128RNA PCR Primer, Index 1 (100% over 32bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA1986020.1931669879502557No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT1916460.1864013482881074No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1738220.16906512612908906No Hit
TGGATTTTTGGAGCAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1645010.1599992078871563RNA PCR Primer, Index 1 (100% over 32bp)
AGGGCTTCTGTCGTGAGTGGCACACGTATGGAATTCTCGGGTGCCAAGGA1621480.15771060091115932RNA PCR Primer, Index 1 (100% over 22bp)
GGTTCGTGCTGAAGGCCTGTATCCTAGGCTTGGAATTCTCGGGTGCCAAG1566350.15234847160445666No Hit
CATAATTTTTTGGTATTTGGGCAGCTGGTTGGAATTCTCGGGTGCCAAGG1559580.15168999862411242Illumina Small RNA Adapter 2 (100% over 21bp)
GTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAGG1529620.1487759882118358Illumina Small RNA Adapter 2 (100% over 21bp)
GGTTCGTGCTGAAGGCCTGTATCCTAGGCTGGAATTCTCGGGTGCCAAGG1522350.1480688835490437Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCA1507660.14664008471872514No Hit
CGGCTCCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTT1439410.14000186006459026RNA PCR Primer, Index 35 (100% over 39bp)
CTTTCCGCCTAGGGGAAAGTCCCCGGACCTTGGAATTCTCGGGTGCCAAG1413950.1375255347943445No Hit
ACCGGCCCAGGTCGGAAACGGAGCAGGTCTGGAATTCTCGGGTGCCAAGG1392060.13539643973677654Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCTGGAATTCTCGGGTGCCAA1369530.13320509612567533No Hit
GCATAATTTTTTGGTATTTGGGCAGCTGGTGGAATTCTCGGGTGCCAAGG1343410.13066457703459836Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG1321870.1285695241547439No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAATGGAATTCTCGGGTGCCAAGGA1312970.12770388020717174RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA1299730.126416113255952No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAAAGAGTGGAATTCTCGGGTGCCA1298750.1263207951583542No Hit
CACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGT1270910.12361298307965653No Hit
AGGAGGGGTGAACCGGCCCAGGTCGGTGGAATTCTCGGGTGCCAAGGAAC1222540.11890835411964916RNA PCR Primer, Index 1 (100% over 24bp)
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCATGGAATTCTCGGGTGCCA1159090.11273699361701386No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCTGGAATTCTCGGGT1126660.10958274269344472No Hit
TGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCA1123590.10928414416321477RNA PCR Primer, Index 1 (100% over 28bp)
GGGAGAGCGGCCGCCAGATCTTCCGGATGGCTCCTGGAATTCTCGGGTGC1097940.10678933885363881No Hit
CACACGGGGCTCATTCTCAGCGCGGCTGTGGAATTCTCGGGTGCCAAGGA1086550.10568150912747623RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1076970.10474972609177495No Hit
ACGCGCCCGACTGCAAACACCACGTGCTGGAATTCTCGGGTGCCAAGGAA1028500.10003537079527798RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)