FastQCFastQC Report
Sat 23 Sept 2023
SRR14243836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14243836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137538262
Total Bases20.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAGG80527725.854932207882633Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAAC29918482.1752841402052905RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT22012331.6004513711246404RNA PCR Primer, Index 1 (100% over 30bp)
CCCTTGTAAATTTAACTGTTAGTCCAAAGTGGAATTCTCGGGTGCCAAGG16893331.2282640302667196Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGATTTTTGGAGCAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC15287361.1114987042660172RNA PCR Primer, Index 1 (100% over 31bp)
CCCTTGTAAATTTAACTGTTAGTCCAATGGAATTCTCGGGTGCCAAGGAA12941220.9409178080205783RNA PCR Primer, Index 1 (100% over 23bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG11642870.8465186218508417No Hit
CATAATTTGTGGTAGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC10193690.7411530327466258RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA9266580.6737456083311566No Hit
GGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAGGA8680610.6311414637477388RNA PCR Primer, Index 1 (100% over 22bp)
ATGGATTTTTGGAGCAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA7841140.5701060843709077RNA PCR Primer, Index 1 (100% over 32bp)
TACCCTGGTAGTCCACGCCGTAAACGATGTGGAATTCTCGGGTGCCAAGG6591280.4792324626001163Illumina Small RNA Adapter 2 (100% over 21bp)
TGGATTTTTGGAGCAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5848320.42521403971209115RNA PCR Primer, Index 1 (100% over 32bp)
CGGCTCCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACA5357520.3895294241830684RNA PCR Primer, Index 36 (100% over 39bp)
CATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5309310.3860242177554926RNA PCR Primer, Index 1 (100% over 32bp)
AAGGCGAGCGAGAAAACCAATGTTATGGAATTCTCGGGTGCCAAGGAACT5127700.37281989211118577RNA PCR Primer, Index 1 (100% over 25bp)
CCCTTGTAAATTTAACTGTTAGTCCAAAGATGGAATTCTCGGGTGCCAAG4942260.35933709850136103No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA4706240.34217678277772623No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGTGGAATTCTCGGGTGCCAAGGA4508170.32777569924505806RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA4474410.32532110955422716RNA PCR Primer, Index 1 (100% over 22bp)
AGGGCTTCTGTCGTGAGTGGCACACGTATGGAATTCTCGGGTGCCAAGGA4310870.31343060013365587RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG4259300.3096810980496467No Hit
TTTATAGGTAGAGGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4096440.29784002941668697RNA PCR Primer, Index 1 (100% over 32bp)
GATTTATAGGTAGAGGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT3948680.28709683709686545RNA PCR Primer, Index 1 (100% over 30bp)
CCCTTGTAAATTTAACTGTTAGTCCAAATGGAATTCTCGGGTGCCAAGGA3778570.27472864241951817RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA3688310.26816610493449455RNA PCR Primer, Index 1 (100% over 22bp)
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA3649610.26535234246307404RNA PCR Primer, Index 1 (100% over 23bp)
AGGCGAGCGAGAAAACCAATGTTATGGAATTCTCGGGTGCCAAGGAACTC3640710.2647052498016879RNA PCR Primer, Index 1 (100% over 26bp)
AGGAGGGGTGAACCGGCCCAGGTCGGTGGAATTCTCGGGTGCCAAGGAAC3587690.26085032250880125RNA PCR Primer, Index 1 (100% over 24bp)
TGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCA3542730.2575814139631923RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTGGAATTCTCGGG3445520.2505135625459627No Hit
CCGGCTCCGGGACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3349200.24351042039487164RNA PCR Primer, Index 1 (100% over 33bp)
CCCTTGTAAATTTAACTGTTAGTCCTGGAATTCTCGGGTGCCAAGGAACT3310190.24067411874086356RNA PCR Primer, Index 1 (100% over 25bp)
CCCTTGTAAATTTAACTGTTAGTCCAAAGAGTGGAATTCTCGGGTGCCAA3284980.23884117424720694No Hit
CTAACCAGGATTCCCTCAGTAACGTGGAATTCTCGGGTGCCAAGGAACTC3230720.23489609022396982RNA PCR Primer, Index 1 (100% over 26bp)
GTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAGG3219520.23408177136919178Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG3145740.22871744591334156No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA3142390.2284738773273142No Hit
CAATTGGACCAATCTATCACCCTTGGAATTCTCGGGTGCCAAGGAACTCC2989310.21734388355147313RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGCCCAGGTCGGAAACGGAGCAGGTCTGGAATTCTCGGGTGCCAAGG2966290.21567016747674186Illumina Small RNA Adapter 2 (100% over 21bp)
CTAGCATCACCAGTATTAGAGGTGGAATTCTCGGGTGCCAAGGAACTCCA2911770.21170617962294738RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTCAGGATGAACGCTCGCTGTGTGCCTTGGAATTCTCGGGTGCCAAG2870550.20870919540920185No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTCGTATGC2658550.1932953028009035RNA PCR Primer, Index 36 (100% over 50bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG2642040.19209490956051198No Hit
GGAGGGGTGAACCGGCCCAGGTCGGTGGAATTCTCGGGTGCCAAGGAACT2580500.18762051828166915RNA PCR Primer, Index 1 (100% over 25bp)
GCCGCGACCGGCTCCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2551910.18554182399076702RNA PCR Primer, Index 1 (100% over 31bp)
CAATTGGACCAATCTATCACCCTGGAATTCTCGGGTGCCAAGGAACTCCA2547090.18519137605505007RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC2475310.17997246468041017No Hit
AGCTCAGGATGAACGCTCGCTGTGTGCCTTGGAATTCTCGGGTGCCAAGG2456410.17859830161297224Illumina Small RNA Adapter 2 (100% over 21bp)
GCATAATTTGTGGTAGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2336640.16989017935968975RNA PCR Primer, Index 1 (100% over 30bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC2265120.1646901718156072No Hit
AATTGGACCAATCTATCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCA2212120.16083669866353265RNA PCR Primer, Index 1 (100% over 28bp)
TGGATTTTTGGAGCAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2208590.16058004281019633RNA PCR Primer, Index 1 (100% over 33bp)
CGCCGCGACCGGCTCCGGGACGTGGAATTCTCGGGTGCCAAGGAACTCCA2199170.15989514248769554RNA PCR Primer, Index 1 (100% over 28bp)
AGCATAATTTTTTGGTATTTGGGCAGCTGGTGGAATTCTCGGGTGCCAAG2195140.15960213311405666No Hit
GGGCTTCTGTCGTGAGTGGCACACGTTGGAATTCTCGGGTGCCAAGGAAC2183380.15874709831653974RNA PCR Primer, Index 1 (100% over 24bp)
AATTGGACCAATCTATCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAG2101340.1527822127052907RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA2082150.15138696459607726No Hit
CATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2043320.1485637502093781RNA PCR Primer, Index 1 (100% over 30bp)
AGGGCTTCTGTCGTGAGTGGCACACGTTGGAATTCTCGGGTGCCAAGGAA2039820.14830927556725998RNA PCR Primer, Index 1 (100% over 23bp)
AAGGCGAGCGAGAAAACCAATGTTAAGGTGGAATTCTCGGGTGCCAAGGA2002730.1456125714312138RNA PCR Primer, Index 1 (100% over 22bp)
ATTTATAGGTAGAGGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1996670.14517196676514643RNA PCR Primer, Index 1 (100% over 31bp)
TGCATAATTTGTGGTAGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG1980610.1440042916930272RNA PCR Primer, Index 1 (100% over 29bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG1974270.14354332905559036Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTAGTCCACGCCGTAAACGATGTGGAATTCTCGGGTGCCAAGGAA1932960.14053980120819035RNA PCR Primer, Index 1 (100% over 23bp)
AGGGGTGAACCGGCCCAGGTCGGTGGAATTCTCGGGTGCCAAGGAACTCC1912690.13906602949512334RNA PCR Primer, Index 1 (100% over 27bp)
AAGGCGAGCGAGAAAACCAATGTTAATGGAATTCTCGGGTGCCAAGGAAC1911250.13896133135665187RNA PCR Primer, Index 1 (100% over 24bp)
CTCTTTGGACACTAGGAAAAAACCTTGTGGAATTCTCGGGTGCCAAGGAA1894950.13777620659478743RNA PCR Primer, Index 1 (100% over 23bp)
CCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAACT1859600.13520601270939428RNA PCR Primer, Index 1 (100% over 25bp)
AACCGGCCCAGGTCGGAAACGGAGCAGGTCTGGAATTCTCGGGTGCCAAG1846080.13422301352041222No Hit
AGGCGGGCATGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACA1826810.1328219488479504RNA PCR Primer, Index 36 (100% over 39bp)
AGGCGAGCGAGAAAACCAATGTTAAGGTGGAATTCTCGGGTGCCAAGGAA1814910.13195673506474875RNA PCR Primer, Index 1 (100% over 23bp)
CCCTTGTAAATTTAACTGTTAGTTGGAATTCTCGGGTGCCAAGGAACTCC1813380.13184549329262282RNA PCR Primer, Index 1 (100% over 27bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAAGTGGAATTCTCGGGTGCCAAG1810010.13160047056578336No Hit
AAGGAGGGGTGAACCGGCCCAGGTCGGTGGAATTCTCGGGTGCCAAGGAA1808900.13151976575071161RNA PCR Primer, Index 1 (100% over 23bp)
AGGAGGGGTGAACCGGCCCAGGTCGGATGGAATTCTCGGGTGCCAAGGAA1795790.13056657644837769RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1784750.1297638907200965No Hit
GGGCTTCTGTCGTGAGTGGCACACGTATGGAATTCTCGGGTGCCAAGGAA1740150.12652115670910544RNA PCR Primer, Index 1 (100% over 23bp)
ACGCGCCCGACTGCAAACACCACGTGCTGGAATTCTCGGGTGCCAAGGAA1730750.1258377105274167RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGAACT1652220.12012802662869189RNA PCR Primer, Index 1 (100% over 25bp)
TTGGACCAATCTATCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1645440.11963507289338875RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG1638580.1191363025948372No Hit
ACCCTGGTAGTCCACGCCGTAAACGATGTGGAATTCTCGGGTGCCAAGGA1623710.11805514890103816RNA PCR Primer, Index 1 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCA1611090.11713758604860079No Hit
CTTTGGACACTAGGAAAAAACCTTGTGGAATTCTCGGGTGCCAAGGAACT1608830.11697326813683308RNA PCR Primer, Index 1 (100% over 25bp)
CATAATTTTTTGGTATTTGGGCAGCTGGTGGAATTCTCGGGTGCCAAGGA1593270.11584194658501645RNA PCR Primer, Index 1 (100% over 22bp)
GCTCTTTGGACACTAGGAAAAAACCTTGTGGAATTCTCGGGTGCCAAGGA1591890.11574161086898131RNA PCR Primer, Index 1 (100% over 22bp)
GAGGCGGGCATGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAAC1565650.11383377812350136RNA PCR Primer, Index 36 (100% over 38bp)
TAACCAGGATTCCCTCAGTAACGTGGAATTCTCGGGTGCCAAGGAACTCC1532620.11143226457231224RNA PCR Primer, Index 1 (100% over 27bp)
TTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAGGA1514300.11010027158842534RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCGGCTCCGGGACGGCTGGTGGAATTCTCGGGTGCCAAGGAACTC1492540.10851816638485659RNA PCR Primer, Index 1 (100% over 26bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGTGGAATTCTCGGGTGCCAAGG1490950.10840256219029436Illumina Small RNA Adapter 2 (100% over 21bp)
GAGGGGTGAACCGGCCCAGGTCGGTGGAATTCTCGGGTGCCAAGGAACTC1442350.10486900001688258RNA PCR Primer, Index 1 (100% over 26bp)
CCTTGTAAATTTAACTGTTAGTCCAAAGTGGAATTCTCGGGTGCCAAGGA1415550.10292045132866373RNA PCR Primer, Index 1 (100% over 22bp)
AGAGGCGGGCATGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAA1411940.10265797891207903RNA PCR Primer, Index 36 (100% over 37bp)
CGCGCCGGGCCCGGGTCTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCC1408130.10238096508737328RNA PCR Primer, Index 1 (100% over 27bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCAA1402280.10195562889983298No Hit
CAATTGGACCAATCTATCACCTGGAATTCTCGGGTGCCAAGGAACTCCAG1396210.10151429716335954RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTTCGCTGTCTCGGCTGCAGACTCGGTGGAATTCTCGGGTGCCAAGG1394060.1013579770260584Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGCGAGCGAGAAAACCAATGTTTGGAATTCTCGGGTGCCAAGGAACTC1383460.10058728239564346RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph