FastQCFastQC Report
Wed 28 Jun 2023
SRR1425203.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1425203.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18782455
Total Bases595.1 Mbp
Sequences flagged as poor quality0
Sequence length5-49
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCA1682890.8959904336254232No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCA1118930.5957314951639708No Hit
AAGGCTGAGGCGTGATGACGAGGCAC853590.45446135768726714No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCA611120.32536747725470394No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCC599480.3191702043210007No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC535070.2848775625976476No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCT531600.2830300937763461No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCA469930.250196260286528No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCA377560.20101738563994961No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCT375530.1999365897589No Hit
TAGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCA372240.1981849550551299No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC353900.1884205233021988No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT334870.1782887274320636No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCT295010.1570667945164783No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCT266830.1420634310051588No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACC253710.135078188660641No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCA251110.13369391807407496No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGA244210.13002027690203438No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGA226430.120553995736979No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCG224930.11975537809088323No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCC222530.11847758985712997No Hit
AGGCACGCTGATATGTAGGTGAAGCGAC220090.11717850515281417No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATG213940.11390417280382145No Hit
TAACCGGGAGATAGCTGGTTCTCCCCGAAAGCT209010.11127938280698663No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA201140.10708930222380407No Hit
ACGTATCGGGGGTTCGAATCCCCCCCTCACCGCCA193170.10284598046421513No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCA191210.10180245340664998No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC190850.10161078517158699No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCA190580.10146703399528975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)