FastQCFastQC Report
Wed 28 Jun 2023
SRR1425204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1425204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28587329
Total Bases869.1 Mbp
Sequences flagged as poor quality0
Sequence length5-48
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCAC4470111.5636682951387308No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCA1268470.4437175645195814No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC978850.3424069453987814No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCC836520.2926191530520392No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCA765120.2676430526265675No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAA752850.2633509412509298No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCG627890.21963926745307336No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC616420.21562700033990584No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCA598920.20950540709836868No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGC581760.2035027476683813No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT542200.18966444888922643No Hit
TAAGGCTGAGGCGTGATGACGAGGCAC497110.17389172664574573No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCA484470.16947018729871546No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA481150.16830883360946383No Hit
AAAGGCTGAGGCGTGATGACGAGGCAC454510.15899001966920379No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATC449320.15717453001642792No Hit
TAGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCA442910.15493227786338484No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCT425070.1486917508102978No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCA397240.13895666852961325No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG396080.13855089434903137No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCA389250.1361617239581914No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC388820.13601130766711364No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACC378970.1325657251854484No Hit
AGGCACGCTGATATGTAGGTGAAGCGAC374280.13092513819671645No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGA355040.12419488368430644No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATG344730.12058839075172081No Hit
ACGATGTCGACTTGGAGGTTGTGCCC331840.11607940007266855No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGA311220.10886641420749732No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCA293630.10271333848643222No Hit
TAGGCTGAGGCGTGATGACGAGGCAC287460.10055503961213025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)